Genomic characterization of four Escherichia coli strains isolated from oral lichen planus biopsies

• Published in: Journal of oral microbiology Vol. 13; no. 1; p. 1905958
• Main Authors: Min, Huitae, Baek, Keumjin, Lee, Ahreum, Seok, Yeong-Jae, Choi, Youngnim
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.01.2021; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Antibiotic resistance; Biopsy; Coliforms; comparative genomics; Disseminated infection; E coli; Escherichia coli; Genomes; Genomics; Inflammatory diseases; Lichen planus; Lymphocytes; Lymphocytes T; Oral cavity; Oral lichen planus; Original; Pathogenesis; Phylogeny; Resistance factors; Strains (organisms); Virulence; virulence factor; Virulence factors; whole genome; Oral lichen planus; virulence factor; antibiotic resistance; Escherichia coli; comparative genomics; whole genome
• ISSN: 2000-2297; 2000-2297
• DOI: 10.1080/20002297.2021.1905958

Oral lichen planus (OLP) is a chronic T cell-mediated inflammatory disease that affects the mucus membrane of the oral cavity. We previously proposed a potential role of intracellular bacteria detected within OLP lesions in the pathogenesis of OLP and isolated four Escherichia coli strains from OLP tissues that were phylogenetically close to K-12 MG1655 strain. We sequenced the genomes of the four OLP-isolated E. coli strains and generated 6.71 Gbp of Illumina MiSeq data (166-195x coverage per strain). The size of the assembled draft genomes was 4.69 Mbp, with a GC content of 50.7%, in which 4360 to 4367 protein-coding sequences per strain were annotated. We also identified 368 virulence factors and 53 antibiotic resistance genes. Comparative genomics revealed that the OLP-isolated strains shared more pangenome orthologous groups with pathogenic strains than did the K-12 MG1655 strain, a derivative of K-12 strain isolated from human feces. Although the OLP-isolated strains did not have the major virulence factors (VFs) of the pathogenic strains, a number of VFs involved in adherence/invasion, colonization, or systemic infection were identified. The genomic characteristics of E. coli first isolated from the oral cavity would benefit future investigations on the pathogenic potential of these bacteria.