Survivability of porcine epidemic diarrhea virus (PEDV) in bovine plasma submitted to spray drying processing and held at different time by temperature storage conditions

• Published in: Veterinary microbiology Vol. 174; no. 3-4; pp. 427 - 432
• Main Authors: Pujols, Joan, Segalés, Joaquim
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.12.2014
• Subjects: Animals; Cattle; Cattle Diseases - virology; Chlorocebus aethiops; Coronavirus Infections - veterinary; Coronavirus Infections - virology; Desiccation; Infectivity; Plasma - virology; Porcine epidemic diarrhea virus; Porcine epidemic diarrhea virus (PEDV); Porcine epidemic diarrhea virus - physiology; Spray dried plasma; Storage; Temperature; Vero Cells; Virus Inactivation; Temperature; Spray dried plasma; Storage; Porcine epidemic diarrhea virus (PEDV); Infectivity
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2014.10.021

•Survivability of PEDV in spray dried bovine plasma (SDBP) was assessed.•PEDV titers (TCID50) and loads expressed (qRT-PCR Ct values) were correlated.•PEDV inoculated on bovine plasma was inactivated at either 70 or 80°C throughout substance.•PEDV lost infectivity when stored at 22°C (7 days), 12°C (14 days) and 4°C (21 days). Bovine plasma was inoculated with porcine epidemic diarrhea virus (PEDV) at an average final titer of 4.2 log10 TCID50/mL to determine the effect of spray drying on viral inactivation. Using a laboratory scale drier, inoculated plasma was spray dried at 200°C inlet temperature and either 70 or 80°C throughout substance. Both liquid and dried samples were subjected to three passages on VERO cell monolayers to determine PEDV infectivity. Results indicated liquid samples contained infective virus, but none of the spray dried samples were infectious. Also, survivability of PEDV inoculated on spray dried bovine plasma (SDBP) and stored at 4, 12 or 22°C was determined for 7, 14 and 21 days. Commercial SDBP powder was inoculated with PEDV to an average final titer of 2.8 log10 TCID50/g. Five samples per time and temperature conditions were subjected to three passages on VERO cell monolayers to determine PEDV infectivity. The virus was non-infectious for all samples stored at 22°C at 7, 14 and 21 days. PEDV was infective in 1 out of 5 samples stored at 12°C at 7 days, but none of the samples stored for 14 and 21 days were infectious in cell culture. For samples stored at 4°C, 4 out of 5 samples were infectious at 7 days, 1 out of 5 samples were infectious at 14 days, but none were infectious at 21 days. In summary, PEDV was not infectious on cell culture within 7 days when stored at room temperature and within 21 days when stored at refrigerated temperature.