A more efficient method to generate integration-free human iPS cells
Human induced pluripotent stem cells are generated with episomal plasmid vectors at increased efficiency using non-transforming L-Myc and knockdown of p53. Also in this issue, Chen et al . report defined conditions for human cell reprogramming and culture. We report a simple method, using p53 suppre...
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Published in | Nature methods Vol. 8; no. 5; pp. 409 - 412 |
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Main Authors | , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Nature Publishing Group US
01.05.2011
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Human induced pluripotent stem cells are generated with episomal plasmid vectors at increased efficiency using non-transforming L-Myc and knockdown of p53. Also in this issue, Chen
et al
. report defined conditions for human cell reprogramming and culture.
We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1548-7091 1548-7105 |
DOI: | 10.1038/nmeth.1591 |