Induction of nitric oxide synthase by cytokines in vascular smooth muscle cells

We investigated the mechanisms by which cytokines lead to a diminished responsiveness of vascular smooth muscle to vasoconstrictors. The attenuation of noradrenaline-induced contraction by 6 to 24 h incubations with the cytokines, tumor necrosis factor and interleukin-1, in endotheliumdenuded rabbi,...

Full description

Saved in:
Bibliographic Details
Published inFEBS letters Vol. 275; no. 1; pp. 87 - 90
Main Authors Busse, Rudi, Mülsch, Alexander
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 26.11.1990
Elsevier
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:We investigated the mechanisms by which cytokines lead to a diminished responsiveness of vascular smooth muscle to vasoconstrictors. The attenuation of noradrenaline-induced contraction by 6 to 24 h incubations with the cytokines, tumor necrosis factor and interleukin-1, in endotheliumdenuded rabbi, aorta was associated with an increase in intracellular cyclic GMP level. This increase was abolished by the stereoselective inhibitor of nitric oxide-synthase. N o-nitro-L-arginine and by cycloheximide. Formation of nitric oxide was detected in the cytosol of cytokine-treated native and cultured smooth muscle cells by activation of purified soluble guanylate cyclase, and depended on tetrahydrobiopterin, but not on ? -calmodulin. The results indicate that cytokines induce a nitric oxide-synthase of the macrophage-type in vascular smooth muscle.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)81445-T