离子液体分散液相微萃取-毛细管电泳法检测尿液中的毒品

利用离子液体分散液相微萃取技术,结合高效毛细管电泳技术,对尿液中痕量麻黄碱和氯胺酮毒品实现高效富集及分离检测。实验中,对影响萃取效率的因素,包括离子液体和分散剂的种类及用量、样品溶液中NaOH的加入量、反萃取试剂HCl的加入量等进行了优化。0.5mL预处理过的样品溶液加入3.5mL0.2g/LNaOH溶液,以40mL[BMIM]PF6为萃取溶剂,350mL乙腈为分散剂萃取数秒后,反萃取通过向富含离子液体的上清液中加入60μL0.1mol/LHCl完成。麻黄碱和氯胺酮在0.10～10mg/L范围内具有良好的线性关系（r2分别为0.9968和0.9981）;富集因子分别为28.8和16.9,检出限...

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Bibliographic Details
Published in分析化学 Vol. 41; no. 12; pp. 1919 - 1922
Main Author 刘心 付饶 李敏 郭黎平 杨丽
Format Journal Article
LanguageChinese
Published 东北师范大学化学学院,长春,130024 2013
Subjects
分散液相微萃取
毒品
毛细管电泳
离子液体
毒品
毛细管电泳
Capillary electrophoresis
Dispersive liquid-liquid microextraction
Drug of abuse
离子液体
分散液相微萃取
Ionic liquid
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Summary:利用离子液体分散液相微萃取技术,结合高效毛细管电泳技术,对尿液中痕量麻黄碱和氯胺酮毒品实现高效富集及分离检测。实验中,对影响萃取效率的因素,包括离子液体和分散剂的种类及用量、样品溶液中NaOH的加入量、反萃取试剂HCl的加入量等进行了优化。0.5mL预处理过的样品溶液加入3.5mL0.2g/LNaOH溶液,以40mL[BMIM]PF6为萃取溶剂,350mL乙腈为分散剂萃取数秒后,反萃取通过向富含离子液体的上清液中加入60μL0.1mol/LHCl完成。麻黄碱和氯胺酮在0.10～10mg/L范围内具有良好的线性关系（r2分别为0.9968和0.9981）;富集因子分别为28.8和16.9,检出限为0.015和0.03mg/L（S/N=3）;重现性RSD〈6.8%（n=6）。本方法成功应用于尿液中麻黄碱和氯胺酮的分析检测,检出限分别为0.21和0.39mg/L,加标回收率为79%～90%。本方法具有价廉、易操作、高富集、检测灵敏及干扰小等特点,可用于生物检材中痕量毒品的检测。
Bibliography:Ionic liquid; Dispersive liquid-liquid microextraetion; Capillary electrophoresis; Drug of abuse
22-1125/O6
A method for dispersive liquid-liquid microextraction using ionic-liquid was applied for preconcentration, separation and simultaneous detection of trace-amount ephedrine and ketamine drugs in urine samples, by coupling with high performance capillary electrophoresis. Various experimental conditions, including selection of ionic-liquid and dispersant and their concentrations, the amount of addition of NaOH and HC1 in the sample, were investigated and optimized. The pretreated sample （0.5 mL） was added into 3.5 mL 0.2 g/L NaOH solution and its extraction was performed within several seconds by using 40 mL [ BMIM ] PF6 as extraction solvent and 350 mL aeetonitrile as dispersant, then the analytes were back-extracted into 60 txL O. 1 mol/L HC1 solution. Under the optimum conditions, the method shows good linearity for detection of ephedrine and ketamine in the range of 0. 10 - 10 mg/L （ r2 is 0. 9968 for eph
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2013.30253