Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research

• Published in: Genome Vol. 49; no. 9; pp. 1057 - 1068
• Main Authors: Jiang, J, Gill, B.S
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.09.2006; Canadian Science Publishing NRC Research Press
• Subjects: Analysis; Bass; biologie du chromosome; chromatin; chromosome biology; Chromosome Painting; Chromosomes; Chromosomes, Plant; Cloning; cytogenetic analysis; cytogénétique moléculaire; Deoxyribonucleic acid; digital images; DNA; Fibers; FISH; Fluorescence; fluorescence in situ hybridization; Forecasts and trends; gene expression regulation; genetic markers; Genetics; genome; genome research; Genome, Plant; genomics; Hybridization; Immunoassay; Immunoassays; In Situ Hybridization, Fluorescence - methods; Karyotyping; literature reviews; meiosis; Methods; molecular cytogenetics; molecular sequence data; nucleotide sequences; Phylogeny; Physical Chromosome Mapping; Plant genetic engineering; Plant species; plants; Proteins; recherche génomique
• ISSN: 0831-2796; 1480-3321
• DOI: 10.1139/g06-076

Fluorescence in situ hybridization (FISH), which allows direct mapping of DNA sequences on chromosomes, has become the most important technique in plant molecular cytogenetics research. Repetitive DNA sequence can generate unique FISH patterns on individual chromosomes for karyotyping and phylogenetic analysis. FISH on meiotic pachytene chromosomes coupled with digital imaging systems has become an efficient method to develop physical maps in plant species. FISH on extended DNA fibers provides a high-resolution mapping approach to analyze large DNA molecules and to characterize large genomic loci. FISH-based physical mapping provides a valuable complementary approach in genome sequencing and map-based cloning research. We expect that FISH will continue to play an important role in relating DNA sequence information to chromosome biology. FISH coupled with immunoassays will be increasingly used to study features of chromatin at the cytological level that control expression and regulation of genes.