Low Mitogenic Activity of Synthetic Lipid A Analog, 2, 3-Acyloxyacylgalactosamine-4-phosphate, in Cultured Murine Splenocytes

• Published in: Biological & pharmaceutical bulletin Vol. 16; no. 2; pp. 201 - 202
• Main Authors: SHIMIZU, Tadayori, IWAMOTO, Yoshihisa, YANAGIHARA, Yasutake, ACHIWA, Kazuo, MASUZAWA, Toshiyuki, IKEDA, Kiyoshi, SANO, Kenji
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1993; Maruzen; Japan Science and Technology Agency
• Subjects: Animals; Biological and medical sciences; Cell Division - drug effects; Cells, Cultured; Lipid A - analogs & derivatives; Lipid A - pharmacology; lipid A analog; lipopolysaccharide; Lipopolysaccharides - pharmacology; Male; Medical sciences; Mice; Mice, Inbred C57BL; Miscellaneous; mitogenicity; Mitogens - pharmacology; Pharmacology. Drug treatments; Salmonella typhimurium - metabolism; Spleen - cytology; Spleen - drug effects; Thymidine - metabolism; Cell culture; Synthetic product; Analog; Animal; Lipopolysaccharide; Lipids; Mitogen; Splenocyte; In vitro
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.16.201

The mitogenicity of chemically synthesized lipid A analogs, 2, 3-acyloxyacylglucosamine-4-phosphate (A-103) and 2, 3-acyloxyacylgalactosamine-4-phosphate (A-113), was compared. Synthetic lipid A analogs of the disaccharide-type (506), the monosaccharide-type (A-103) suspended in RPMI-1640 medium supplemented with triethylamine, and Salmonella typhimurium LT-2 lipopolysaccharide (LPS) were copable of increasing the incorporation of [3H]thymidine into splenocytes of C57BL/6 mice at various doses ranging from 1.0 to 100 μg/ml. However, the mitogenic activity of A-113 at these doses was markedly weaker than the activity of the above materials. When the A-103 and A-113 were suspended in RPMI-1640 medium supplemented with ethanol, the mitogenic activity of A-113 also showed lower activity than that of A-103 in the splenocytes of C57BL/6 mice. These findings indicate that the mitogenic activity of synthetic lipid A of the monosaccharide-type is affected by a kind of composed sugar.