Anti-feline panleukopenia virus serum neutralizing antibody titer in domestic cats with the negative or low hemagglutination inhibition antibody titer

• Published in: Journal of Veterinary Medical Science Vol. 81; no. 2; pp. 252 - 255
• Main Authors: SOMA, Takehisa, OHTA, Kaisaku, YAMASHITA, Runa, SASAI, Kazumi
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2019; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: cat; Feline panleukopenia; feline panleukopenia virus; Hemagglutination inhibition; hemagglutination inhibition test; serum neutralizing test; Virology; hemagglutination inhibition test; feline panleukopenia virus; serum neutralizing test; cat
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.18-0472

To evaluate the accuracy of hemagglutination inhibition (HI) test as the index of feline panleukopenia virus (FPV)-protective ability, sera from 153 FPV-vaccinated cats aged ≥7 months with HI titer of <1:10–1:40, were examined for serum neutralizing (SN) antibody. SN antibody was detected (≥1:10) in 33 (62.3%) of 53 HI antibody-negative cats, and ranged <1:10–1:160. This suggests that FPV-antibody detection sensitivity of HI test is lower than SN test, and SN test is more suitable for the assessment of FPV-vaccine effect than HI test especially in cats with negative or low HI titer. SN titer was 1:32, FPV-protective threshold, or higher in all cats with HI titers of ≥1:20, suggesting it may be appropriate to set protective HI threshold at 1:20.