Gln3 Phosphorylation and Intracellular Localization in Nutrient Limitation and Starvation Differ from Those Generated by Rapamycin Inhibition of Tor1/2 in Saccharomyces cerevisiae

• Published in: The Journal of biological chemistry Vol. 279; no. 11; pp. 10270 - 10278
• Main Authors: Cox, Kathleen H., Kulkarni, Ajit, Tate, Jennifer J., Cooper, Terrance G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.03.2004; American Society for Biochemistry and Molecular Biology
• Subjects: Alkaline Phosphatase - metabolism; Ammonium Sulfate - pharmacology; Antifungal Agents - pharmacology; Blotting, Northern; Blotting, Western; Cell Cycle Proteins; Cytoplasm - metabolism; Fluorescent Antibody Technique, Indirect; Gene Deletion; Gln3 protein; Nitrogen - metabolism; Phosphatidylinositol 3-Kinases - metabolism; Phosphoric Monoester Hydrolases - metabolism; Phosphorylation; Phosphotransferases (Alcohol Group Acceptor) - metabolism; rapamycin; Repressor Proteins - biosynthesis; Repressor Proteins - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - biosynthesis; Saccharomyces cerevisiae Proteins - metabolism; Sirolimus - pharmacology; Time Factors; Tor1 protein; Tor2 protein; Transcription Factors - biosynthesis; Transcription Factors - metabolism; Transcription, Genetic; Transcriptional Activation
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M312023200

The ability of the cell to sense environmental conditions and alter gene expression in response to them is critical to its survival. In Saccharomyces cerevisiae, the Tor1/2 serine/threonine kinases are global regulators situated at the top of a signal cascade reported to receive and transmit nutritional signals associated with the nitrogen supply of the cell. At the other end of that cascade is Gln3, one of two transcriptional activators responsible for most nitrogen catabolic gene expression. When nitrogen is in excess, Tor1/2 are active, and Gln3 is phosphorylated and localizes to the cytoplasm. If Tor1/2 are inhibited by rapamycin or mutation, Gln3 becomes dephosphorylated, accumulates in the nucleus, and mediates nitrogen catabolite repression (NCR)-sensitive transcription. The observations that Gln3 also accumulates in the nuclei of cells provided with poor nitrogen sources or during nitrogen starvation has led to the conclusion that Tor1/2 control intracellular Gln3 localization and NCR-sensitive transcription by regulating Gln3 phosphorylation/dephosphorylation. To test this model, we compared Gln3 phosphorylation states and intracellular localizations under a variety of physiological conditions known to elicit different levels of NCR-sensitive transcription. Our data indicate that: (i) observable Gln3 phosphorylation levels do not correlate in a consistent way with the quality or quantity of the nitrogen source provided, the intracellular localization of Gln3, or the capacity to support NCR-sensitive transcription. (ii) Gln3-Myc13 is hyperphosphorylated during nitrogen and carbon starvation, but this uniform response does not correlate with Gln3 intracellular localization. (iii) Gln3-Myc13 dephosphorylation and nuclear localization correlate with one another at early but not late times after rapamycin treatment. These data suggest that rapamycin treatment and growth with poor nitrogen sources bring about nuclear accumulation of Gln3 but likely do so by different mechanisms or by a common mechanism involving molecules other than Gln3 and/or other than the levels of Gln3-Myc13 phosphorylation thus far detected by others and ourselves.