Highly Selective Needle-Type Glucose Sensors Prepared by the Immobilization of Glucose Oxidase on γ-Polyglutamic Acid Film

• Published in: Analytical Sciences Vol. 27; no. 3; p. 337
• Main Authors: YASUZAWA, Mikito, EDAGAWA, Kazuaki, MATSUNAGA, Takuya, TAKAOKA, Hiroki, YABUTANI, Tomoki
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2011; Springer Nature Singapore
• Subjects: Analytical Chemistry; Animals; Biocompatible Materials - chemistry; Biosensing Techniques - instrumentation; Blood Glucose - analysis; Cellulose - analogs & derivatives; Cellulose - chemistry; Chemistry; Dimethylpolysiloxanes - chemistry; Electrodes; Enzymes, Immobilized - chemistry; Glucose - analysis; Glucose Oxidase - chemistry; Horses; Membranes, Artificial; Needles; Polyglutamic Acid - chemistry; Polyurethanes - chemistry
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.27.337

Fine needle-type glucose sensors with an outer diameter of less that 0.2 mm were fabricated using a low-cost biodegradable γ-polyglutamic acid (PGA) not only as a glucose oxidase (GOx) immobilizing material, but also as a permselective inner membrane. PGA film was prepared on a cellulose acetate-coated Pt–Ir wire electrode, and GOx was covalently immobilized on its surface using water-soluble carbodiimide. The obtained electrode was practically not affected by the existence of electroactive compounds, and provided long-term stability for approximately one month. It also functioned in horse serum with a good linear relationship between the current and glucose concentrations within the range of 2.8 to 22.4 mM.