Frequency analysis of HLA class I alleles in Iranian patients with progressive and non-progressive chronic lymphocytic leukemia

• Published in: Human immunology Vol. 75; no. 2; pp. 170 - 175
• Main Authors: Hojjat-Farsangi, M, Razavi, S.M, Sharifian, R.A, Shokri, F
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2014
• Subjects: ADP-ribosyl Cyclase 1 - metabolism; Adult; Allergy and Immunology; B-Lymphocytes - immunology; Disease Progression; Ethnic Groups; Female; Gene Frequency; Genotype; Haplotypes; Histocompatibility Testing; HLA-A Antigens - genetics; HLA-B Antigens - genetics; Humans; Iran; Leukemia, Lymphocytic, Chronic, B-Cell - genetics; Leukemia, Lymphocytic, Chronic, B-Cell - immunology; Male; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; Middle Aged; Young Adult
• ISSN: 0198-8859; 1879-1166; 1879-1166
• DOI: 10.1016/j.humimm.2013.11.003

Abstract Chronic lymphocytic leukemia (CLL) is a malignant disorder of B cell origin, with low incidence in Asian populations. In this study we investigated the HLA-class I A and B allele frequencies in 87 Iranian CLL patients and 64 healthy controls using sequence specific primer-polymerase chain reaction (SSP-PCR) technique. Our results showed increased frequencies of HLA-A11:01 ( p = 0.02) and HLA-B35:01 ( p = 0.002) alleles and HLA-A11:01/B35:01 haplotype ( p = 0.036) and decreased frequencies of HLA-A01:01 ( p = 0.02), HLA-A26:01 ( p = 0.03), HLA-B65:01 ( p = 0.03) and HLA-B53:01 ( p < 0.00001) alleles in CLL patients compared to the control group. Classification of the patients into non-progressive and progressive groups did not reveal significant differences for the frequency of any of the HLA-A and -B alleles or haplotypes between these two subtypes. Comparison between patients with immunoglobulin heavy chain variable region genes (IGHV) mutated ( n = 56) and unmutated ( n = 31) subtypes showed a significant increase in HLA-A32:01 ( p = 0.05) and HLA-A33:01 ( p = 0.05) alleles in IGHV unmutated patients compared to IGHV mutated patients. Similarly, a higher frequency of HLA-B52:01 ( p = 0.037) alleles was observed in CD38+ compared with CD38− patients. Our results obtained from an Iranian population indicate that CLL is associated with distinct HLA class I alleles and haplotypes some of which are linked to disease prognostic factors.