CD133⁺ Neural Stem Cells in the Ependyma of Mammalian Postnatal Forebrain

The postnatal forebrain subventricular zone (SVZ) harbors stem cells that give rise to olfactory bulb interneurons throughout life. The identity of stem cells in the adult SVZ has been extensively debated. Although, ependymal cells were once suggested to have stem cell characteristics, subsequent st...

Full description

Saved in:
Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 105; no. 3; pp. 1026 - 1031
Main Authors Coskun, Volkan, Wu, Hao, Blanchi, Bruno, Tsao, Sean, Kim, Kevin, Zhao, Jing, Biancotti, Juan Carlos, Hutnick, Leah, Krueger, Richard C., Fan, Guoping, de Vellis, Jean, Sun, Yi E.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 22.01.2008
National Acad Sciences
SeriesFrom the Cover
Subjects
AC133 Antigen
Adult stem cells
Aging - physiology
Animals
Animals, Newborn
Antigens, CD - metabolism
Biological Sciences
Brain
Cell lines
Cell Proliferation
Cells, Cultured
Ependyma
Ependyma - cytology
Ependyma - metabolism
Forebrain
Gene expression
Genetic markers
Glycoproteins - metabolism
Lateral ventricles
Mammals
Mice
Multipotent stem cells
Neural stem cells
Neuroglia
Neurons
Neurons - metabolism
Neurosciences
Olfactory Bulb - metabolism
Peptides - metabolism
Prosencephalon - metabolism
Stem cells
Stem Cells - metabolism
Online AccessGet full text

Cover

More Information
Summary:The postnatal forebrain subventricular zone (SVZ) harbors stem cells that give rise to olfactory bulb interneurons throughout life. The identity of stem cells in the adult SVZ has been extensively debated. Although, ependymal cells were once suggested to have stem cell characteristics, subsequent studies have challenged the initial report and postulated that subependymal GFAP⁺ cells were the stem cells. Here, we report that, in the adult mouse forebrain, immunoreactivity for a neural stem cell marker, prominin-1/CD133, is exclusively localized to the ependyma, although not all ependymal cells are CD133⁺. Using transplantation and genetic lineage tracing approaches, we demonstrate that CD133⁺ ependymal cells continuously produce new neurons destined to olfactory bulb. Collectively, our data indicate that, compared with GFAP expressing adult neural stem cells, CD133⁺ ependymal cells represent an additional-perhaps more quiescent-stem cell population in the mammalian forebrain.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Present address: Division of Neonatology, University of New Mexico, University of New Mexico Children's Hospital MSC10 5590, 1 University of New Mexico, Albuquerque, NM 87131-0001.
Edited by Thomas C. Südhof, University of Texas Southwestern Medical Center, Dallas, TX, and approved November 28, 2007
Author contributions: V.C. and Y.E.S. designed research; V.C., H.W., B.B., S.T., K.K., J.Z., and J.C.B. performed research; L.H., R.C.K., G.F., and J.d.V. contributed new reagents/analytic tools; V.C., H.W., B.B., and Y.E.S. analyzed data; and V.C. and Y.E.S. wrote the paper.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0710000105