Single nucleotide polymorphism genotyping of Erysipelothrix rhusiopathiae isolates from pigs affected with chronic erysipelas in Japan

Over the past decades, Erysipelothrix rhusiopathiae strains displaying similar phenotypic and genetic profiles of the attenuated, acriflavine-resistant E. rhusiopathiae Koganei 65-0.15 strain (serovar 1a) have been frequently isolated from pigs affected with chronic erysipelas in Japan. In this stud...

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Published inJournal of Veterinary Medical Science Vol. 79; no. 4; pp. 699 - 701
Main Authors SHIRAIWA, Kazumasa, OGAWA, Yohsuke, NISHIKAWA, Sayaka, KUSUMOTO, Masahiro, EGUCHI, Masahiro, SHIMOJI, Yoshihiro
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.04.2017
Japan Science and Technology Agency
The Japanese Society of Veterinary Science
Subjects
Acriflavine
Animals
Bacterial Vaccines - genetics
Bacteriology
Chronic Disease
Chronic illnesses
Erysipelas
Erysipelas - epidemiology
Erysipelas - microbiology
Erysipelas - veterinary
Erysipelothrix - genetics
Erysipelothrix rhusiopathiae
Farms
Genomes
Genotype
Genotyping
Japan - epidemiology
Koganei 65-0.15 strain
Polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
Polymorphism, Single Nucleotide
Single-nucleotide polymorphism
SNP-based PCR
strain-specific detection
Swine
Swine Diseases - epidemiology
Swine Diseases - microbiology
vaccine
Vaccines
Vaccines, Attenuated - genetics
Japan
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Summary:Over the past decades, Erysipelothrix rhusiopathiae strains displaying similar phenotypic and genetic profiles of the attenuated, acriflavine-resistant E. rhusiopathiae Koganei 65-0.15 strain (serovar 1a) have been frequently isolated from pigs affected with chronic erysipelas in Japan. In this study, using the conventional PCR assay that was designed to detect strain-specific single nucleotide polymorphism (SNP) sites found in the genome of the vaccine strain, we analyzed E. rhusiopathiae isolates from pigs with chronic disease in farms where the Koganei vaccine was used. Out of a total of 155 isolates, 101 isolates (65.2%) were determined to be the vaccine strain by SNP-based PCR. Among the 101 PCR-positive isolates, four isolates were found to be sensitive to acriflavine.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.17-0040