Ultrasound-assisted extraction of flaxseed oil using immobilized enzymes

• Published in: Bioresource technology Vol. 102; no. 21; pp. 9991 - 9996
• Main Authors: Long, Jing-jing, Fu, Yu-jie, Zu, Yuan-gang, Li, Ji, Wang, Wei, Gu, Cheng-bo, Luo, Meng
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2011; Elsevier
• Subjects: analysis; Antioxidant activity; Aqueous enzymatic process; Biological and medical sciences; Biotechnology; Biphenyl Compounds; Biphenyl Compounds - metabolism; Cellulase; Cellulase - metabolism; chemistry; drug effects; endo-1,4-beta-glucanase; Enzyme Stability; Enzyme Stability - drug effects; Enzymes, Immobilized; Enzymes, Immobilized - metabolism; fatty acid composition; Fatty Acids; Fatty Acids - analysis; Flaxseed oil; Free Radical Scavengers; Free Radical Scavengers - pharmacology; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Glycoside Hydrolases; Glycoside Hydrolases - metabolism; Grounds; Hemicellulases; Hydrogen-Ion Concentration; Hydrogen-Ion Concentration - drug effects; Immobilization of enzymes and other molecules; Immobilization techniques; Immobilized enzymes; isolation & purification; Kinetics; linseed; linseed oil; Linseed Oil - analysis; Linseed Oil - chemistry; Linseed Oil - isolation & purification; Linseed Oil - pharmacology; Linum usitatissimum; metabolism; methods; Methods. Procedures. Technologies; pharmacology; Picrates; Picrates - metabolism; polygalacturonase; Polygalacturonase - metabolism; Principal Component Analysis; Scavenging; Solvent extraction; Temperature; Time Factors; ultrasonics; Ultrasonics - methods; Ultrasound; Ultrasound extraction; Unsaturated; Immobilized enzymes; Aqueous enzymatic process; Flaxseed oil; Ultrasound extraction; Antioxidant activity; Aqueous medium; Linseed oil; Ultrasound assisted process; Antioxidant; Immobilized enzyme; Ultrasound
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2011.07.104

► We set up an effective and clean way to extract flaxseed oil. ► Aqueous enzymatic process assisted by ultrasound extraction method was optimized. ► Kinetic parameters of the free and immobilized enzymes were studied. ► Oil shows good quality. An aqueous enzymatic process assisted by ultrasound extraction (AEP-UE) was applied to the extraction of oil from flaxseed (Linum usitatissimum L.). The highest oil recovery of 68.1% was obtained when ground flaxseed was incubated with 130U/g of cellulase, pectinase, and hemicellulase for 12h, at 45°C and pH 5.0. The IC50 values of oil obtained by AEP-UE and organic solvent extraction (OSE), as measured by DPPH scavenging activity essay, were 2.27mg/mL and 3.31mg/mL. The AEP-UE-derived oil had a 1.5% higher content of unsaturated fatty acids than the OSE-derived oil. AEP-UE is therefore a promising environmentally friendly method for large-scale preparation of flaxseed oil.