An Autism-Associated Mutation Impairs Neuroligin-4 Glycosylation and Enhances Excitatory Synaptic Transmission in Human Neurons

• Published in: The Journal of neuroscience Vol. 41; no. 3; pp. 392 - 407
• Main Authors: Cast, Thomas P, Boesch, Daniel J, Smyth, Kim, Shaw, Alisa E, Ghebrial, Michael, Chanda, Soham
• Format: Journal Article
• Language: English
• Published: United States Society for Neuroscience 20.01.2021
• Subjects: Amino Acid Substitution; Amino acids; Autism; Autistic Disorder - genetics; Autistic Disorder - psychology; Cell adhesion; Cell adhesion & migration; Cell adhesion molecules; Cell Adhesion Molecules, Neuronal - genetics; Cell lines; Child; Conserved sequence; Endoplasmic reticulum; Endoplasmic Reticulum - metabolism; Excitatory Postsynaptic Potentials - physiology; Glycosylation; Golgi apparatus; Golgi Apparatus - metabolism; HEK293 Cells; Humans; Localization; Male; Mutation; Mutation - genetics; Mutation, Missense - genetics; Neural Stem Cells; Neurodevelopmental disorders; Neurons; Neuropsychological Tests; Patch-Clamp Techniques; Phenotypes; Stem cells; Substitutes; Synapses; Synapses - metabolism; Synaptic transmission; Synaptic Transmission - physiology; Synaptogenesis; glycosylation; AMPA receptor; synaptic transmission; neuroligin-4; trafficking; autism
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/jneurosci.0404-20.2020

Neuroligins (NLGNs) are a class of postsynaptic cell adhesion molecules that interact with presynaptic neurexins (NRXNs) and regulate synapse function. NLGN4 is a member of the NLGN family and consists of a unique amino acid sequence in humans that is not evolutionarily well conserved in rodents. The human-specific NLGN4 gene has been reported to be mutated in many patients with autism and other neurodevelopmental disorders. However, it remained unclear how these mutations might alter the molecular properties of NLGN4 and affect synaptic transmission in human neurons. Here, we describe a severely autistic male patient carrying a single amino acid substitution (R101Q) in the NLGN4 gene. When expressed in HEK293 cells, the R101Q mutation in NLGN4 did not affect its binding affinity for NRXNs or its capacity to form homodimers. This mutation, however, impaired the maturation of NLGN4 protein by inhibiting N-linked glycosylation at an adjacent residue (N102), which is conserved in all NLGNs. As a result, the R101Q substitution significantly decreased the surface trafficking of NLGN4 and increased its retention in the endoplasmic reticulum and Golgi apparatus. In human neurons derived from male stem cell lines, the R101Q mutation also similarly reduced the synaptic localization of NLGN4, resulting in a loss-of-function phenotype. This mutation-induced trafficking defect substantially diminished the ability of NLGN4 to form excitatory synapses and modulate their functional properties. Viewed together, our findings suggest that the R101Q mutation is pathogenic for NLGN4 and can lead to synaptic dysfunction in autism.