The human growth hormone gene contains both positive and negative control elements

• Published in: The Journal of biological chemistry Vol. 263; no. 11; pp. 5005 - 5007
• Main Authors: Peritz, L N, Fodor, E J, Silversides, D W, Cattini, P A, Baxter, J D, Eberhardt, N L
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 15.04.1988; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Base Sequence; Binding Sites; Biological and medical sciences; Biotechnology; Deoxyribonuclease I - metabolism; DNA - analysis; DNA-Binding Proteins - analysis; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation; Genetic engineering; Genetic technics; Growth Hormone - genetics; HeLa Cells - analysis; Humans; Methods. Procedures. Technologies; Molecular and cellular biology; Molecular genetics; Pituitary Gland, Anterior - analysis; Rats; Transcription, Genetic; Transfection; Tumor Cells, Cultured - analysis; Vectors (cloning, transfer, expression). Insertion sequences and transposons; Human; Hormone; Plasmid; Cell culture; Regulation(control); Transfection; Transcription; STH; DNA binding protein; Gene expression; Hela cell line
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)60666-0

A subset of DNA sequences in the 5'-flanking DNA of the human growth hormone (hGH) gene was examined by protein-DNA binding and gene transfer-expression experiments. Two adjacent cis-acting elements (I and II) were identified between nucleotides -308/-235 of the hGH gene that modulated the expression of a linked reporter gene in transfected HeLa cells. Elements I and II repressed gene expression whereas element II alone activated it. HeLa whole cell extracts contain two factors that bind hGH DNA carrying elements I and II. Factor I binds to single-stranded DNA, and its binding is correlated with repression of gene expression. Factor II binds between nucleotides -275/-257 of the hGH gene. This region is homologous to the binding site for the adenovirus major late transcription factor, and factor II binding to hGH DNA is competed by adenovirus major late promoter DNA, indicating that the hGH and major late adenovirus promoters share a transcription regulatory element.