应用表面增强拉曼光谱技术快速检测尿样中的β-兴奋剂

应用表面增强拉曼光谱技术与化学计量法相结合分析克伦特罗、沙丁胺醇和莱克多巴胺3种β-兴奋剂的标准溶液。在取自10头猪的尿样中,分别添加5个不同浓度的莱克多巴胺（1～20mg/L）,采用快速的液液萃取法对样品进行前处理,再进行表面增强拉曼测试。结果表明,克伦特罗和沙丁胺醇标准溶液的最低检测浓度为2μg/L,莱克多巴胺标准溶液的最低检测浓度为0.1mg/L;通过偏最小二乘法建立模型进行定量分析,3种药物的实际值与预测值的相关系数（R2）为0.9134～0.9368;本方法可检测尿样中1mg/L莱克多巴胺,经外部验证后模型的实际值与预测值的相关系数（R2）为0.881,相对分析误差（RPD）为2.8...

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Bibliographic Details
Published in分析化学 Vol. 40; no. 5; pp. 718 - 723
Main Author 翟福丽 黄轶群 王锡昌 赖克强
Format Journal Article
LanguageChinese
Published 上海海洋大学食品学院,上海,201306 2012
Subjects
β-兴奋剂
尿样
纳米基底
表面增强拉曼光谱
表面增强拉曼光谱
尿样
β-兴奋剂
纳米基底
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Summary:应用表面增强拉曼光谱技术与化学计量法相结合分析克伦特罗、沙丁胺醇和莱克多巴胺3种β-兴奋剂的标准溶液。在取自10头猪的尿样中,分别添加5个不同浓度的莱克多巴胺（1～20mg/L）,采用快速的液液萃取法对样品进行前处理,再进行表面增强拉曼测试。结果表明,克伦特罗和沙丁胺醇标准溶液的最低检测浓度为2μg/L,莱克多巴胺标准溶液的最低检测浓度为0.1mg/L;通过偏最小二乘法建立模型进行定量分析,3种药物的实际值与预测值的相关系数（R2）为0.9134～0.9368;本方法可检测尿样中1mg/L莱克多巴胺,经外部验证后模型的实际值与预测值的相关系数（R2）为0.881,相对分析误差（RPD）为2.83;分析尿液中的莱克多巴胺含量所需时间小于30min,为快速检测莱克多巴胺提供新途径。
Bibliography:ZHAI Fu-Li,HUANG Yi-Qun,WANG Xi-Chang,LAI Ke-Qiang（College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China）
22-1125/O6
Surface enhanced Raman spectroscopy（SERS） and partial least squares（PLS） method was used for the determination of β-agonists in swine urine.Ractopamine was added to urine（1 mg/L to 20 mg/L） for each of the samples collected from ten different swine.A rapid liquid-liquid extraction（LLE） was used to extract ractopamine before SERS analysis.For standard solutions of β-agonists,the characteristic bands of SERS spectra could be detected at concentration as low as 2 μg/L for clenbuteroland salbulamol,and 0.1 mg/L for ractopmiane,the R2 of actual values vs.predicted values based on PLS regression model was 0.9134-0.9368.The results showed that this method could be used to determine the ractopamine at concentration as low as 1 mg/L.For the quantitative analyses,the R2 of actual values vs.their values predicted by the PLS models was 0.881.It took less than 30 min for LLE.O
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2012.10801