Characterization of anamnestic T-cell responses induced by conventional vaccines against contagious bovine pleuropneumonia

• Published in: PloS one Vol. 8; no. 2; p. e57509
• Main Authors: Totte, Philippe, Yaya, Aboubakar, Sery, Amadou, Wesonga, Hezron, Wade, Abel, Naessens, Jan, Niang, Mamadou, Thiaucourt, François
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.02.2013; Public Library of Science (PLoS)
• Subjects: Animals; Bacterial Vaccines - immunology; Bacterial Vaccines - therapeutic use; Biology; Bovidae; Cattle; Cattle Diseases - immunology; Cattle Diseases - prevention & control; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; Comparative analysis; Contagious bovine pleuropneumonia; Data processing; Enzyme-Linked Immunosorbent Assay; Enzymes; Fisheries; Flow Cytometry; Immunity; Immunoglobulins; Immunologic Memory; Infections; Interferon; L-selectin; Life Sciences; Livestock; Lymphocytes; Lymphocytes T; Medicine; Memory cells; Mycobacterium bovis; Mycoplasma; Pleuropneumonia; Pleuropneumonia, Contagious - immunology; Pleuropneumonia, Contagious - prevention & control; Studies; Vaccines; Veterinary Science; γ-Interferon; France
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0057509

A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP). We show here that mycoplasmas-induced recall proliferation and IFN-γ responses are detected in cattle that received multiple shots of T1 vaccines. These anamnestic responses were under the strict control of CD4(+) T lymphocytes. Moreover, CD62L expression indicated that both CD4(+) effector memory (Tem) and central memory (Tcm) T lymphocytes are elicited in these animals. Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment. In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.