Cycloartocarpin Inhibits Migration through the Suppression of Epithelial-to-Mesenchymal Transition and FAK/AKT Signaling in Non-Small-Cell Lung Cancer Cells

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 23; p. 8121
• Main Authors: Tungsukruthai, Sucharat, Sritularak, Boonchoo, Chanvorachote, Pithi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.11.2022; MDPI
• Subjects: AKT protein; Anchorages; Apoptosis; Bioflavonoids; Cadherin; Carcinoma, Non-Small-Cell Lung - metabolism; Cdc42 protein; Cell adhesion & migration; Cell differentiation; Cell division; Cell growth; Cell Line, Tumor; Cell migration; Cell Movement; Cell viability; Cellular signal transduction; cycloartocarpin; Cytotoxicity; Development and progression; Drug therapy; Epithelial-Mesenchymal Transition; epithelial–mesenchymal transition (EMT); Flavones; Flavonoids; Focal adhesion kinase; Health aspects; Humans; Kinases; Lung cancer; Lung cancer, Non-small cell; Lung Neoplasms - metabolism; Medical prognosis; Mesenchyme; Metastases; Metastasis; migration; Motility; N-Cadherin; Pharmacology, Experimental; Phosphorylation; Protein kinases; Protein-tyrosine kinase; Proteins; R&D; Research & development; Signal Transduction; Tyrosine; Vimentin; Wound healing; Thailand; lung cancer; migration; epithelial–mesenchymal transition (EMT); metastasis; cycloartocarpin
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27238121

Lung cancer metastasis is a multifaceted process that accounts for 90% of cancer deaths. According to several studies, the epithelial-mesenchymal transition (EMT) plays an essential role in lung cancer metastasis. Therefore, this study aimed to investigate the potential pharmacological effect of cycloartocarpin on the suppression of metastasis-related behaviors and EMT. An MTT assay was used to examine cell viability. Cell migration was determined using a wound healing assay. Anchorage-independent cell growth was also performed. Western blot analysis was used to identify the key signaling proteins involved in the regulation of EMT and migration. The results found that non-toxic concentrations of cycloartocarpin (10-20 μM) effectively suppressed cell migration and attenuated anchorage-independent growth in H292, A549, and H460 cells. Interestingly, these effects were consistent with the findings of Western blot analysis, which revealed that the level of phosphorylated focal adhesion kinase (p-FAK), phosphorylated ATP-dependent tyrosine kinase (p-AKT), and cell division cycle 42 (Cdc42) were significantly reduced, resulting in the inhibition of the EMT process, as evidenced by decreased N-cadherin, vimentin, and slug expression. Taken together, the results suggest that cycloartocarpin inhibits EMT by suppressing the FAK/AKT signaling pathway, which is involved in Cdc42 attenuation. Our findings demonstrated that cycloartocarpin has antimetastatic potential for further research and development in lung cancer therapy.