Gelatin Methacryloyl (GelMA) Hydrogel Scaffolds: Predicting Physical Properties Using an Experimental Design Approach

There is a growing interest for complex in vitro environments that closely mimic the extracellular matrix and allow cells to grow in microenvironments that are closer to the one in vivo. Protein-based matrices and especially hydrogels can answer this need, thanks to their similarity with the cell mi...

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Published inInternational journal of molecular sciences Vol. 24; no. 17; p. 13359
Main Authors Peyret, Corentin, Elkhoury, Kamil, Bouguet-Bonnet, Sabine, Poinsignon, Sophie, Boulogne, Corentin, Giraud, Tristan, Stefan, Loïc, Tahri, Yasmina, Sanchez-Gonzalez, Laura, Linder, Michel, Tamayol, Ali, Kahn, Cyril J.F, Arab-Tehrany, Elmira
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.09.2023
MDPI
Subjects
Amino acids
Biotechnology
Chemical Sciences
compression
Design of experiments
experimental design
gelatin methacryloyl
Hydrogels
Life Sciences
Material chemistry
multiscale characterisation
NMR
Physical properties
rheology
Tissue engineering
France
Experimental design DOE
NMR
Gelatin
Rheology
Methacryloyl
multiscale characterization
compression
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Summary:There is a growing interest for complex in vitro environments that closely mimic the extracellular matrix and allow cells to grow in microenvironments that are closer to the one in vivo. Protein-based matrices and especially hydrogels can answer this need, thanks to their similarity with the cell microenvironment and their ease of customization. In this study, an experimental design was conducted to study the influence of synthesis parameters on the physical properties of gelatin methacryloyl (GelMA). Temperature, ratio of methacrylic anhydride over gelatin, rate of addition, and stirring speed of the reaction were studied using a Doehlert matrix. Their impact on the following parameters was analyzed: degree of substitution, mass swelling ratio, storage modulus (log(G’)), and compression modulus. This study highlights that the most impactful parameter was the ratio of methacrylic anhydride over gelatin. Although, temperature affected the degree of substitution, and methacrylic anhydride addition flow rate impacted the gel’s physical properties, namely, its storage modulus and compression modulus. Moreover, this experimental design proposed a theoretical model that described the variation of GelMA’s physical characteristics as a function of synthesis conditions.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241713359