Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek's disease virus (MDV) transformed cell line MSB1 by targeting RORA

• Published in: BMC veterinary research Vol. 16; no. 1; p. 23
• Main Authors: Ding, Ke, Yu, Zu-Hua, Yu, Chuan, Jia, Yan-Yan, He, Lei, Liao, Cheng-Shui, Li, Jing, Zhang, Chun-Jie, Li, Yin-Ju, Wu, Ting-Cai, Cheng, Xiang-Chao, Zhou, Zi-Yu, Yu, Zu-Ling
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 28.01.2020; BioMed Central; BMC
• Subjects: Apoptosis; Cancer; Cell cycle; Cell growth; Cell lines; Cell proliferation; Development and progression; Disease; Diseases; G1 phase; Gene expression; Genes; Genetic aspects; Genetic engineering; Gga-miR-155; Health aspects; Infection; Invasiveness; Lymphocyte line; Lymphocytes; Lymphoma; Marek's disease; Metabolism; MicroRNA; MicroRNAs; miRNA; MSB1; Neoplasia; Nuclear receptors; Proliferation; Tumorigenesis; Tumors; Veterinary research; China; United States > US; MSB1; Gga-miR-155; Lymphocyte line; RORA; Marek’s disease; Proliferation; Apoptosis
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-020-2239-4

Marek's disease (MD) is caused by the oncogenic Marek's disease virus (MDV), and is a highly contagious avian infection with a complex underlying pathology that involves lymphoproliferative neoplasm formation. MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms. The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells. The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.