Longitudinal study of Senecavirus a shedding in sows and piglets on a single United States farm during an outbreak of vesicular disease

• Published in: BMC veterinary research Vol. 13; no. 1; p. 277
• Main Authors: Tousignant, Steven J P, Bruner, Laura, Schwartz, Jake, Vannucci, Fabio, Rossow, Stephanie, Marthaler, Douglas G
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 31.08.2017; BioMed Central; BMC
• Subjects: Agriculture; Animal diseases; Animals; Care and treatment; Case reports; Diagnosis; Disease Outbreaks - veterinary; Epidemics; Epidemiology; Farms; Female; Foot & mouth disease; Genomes; Hogs; Infections; Laboratories; Lesions; Longitudinal Studies; Minnesota; Outbreaks; Picornaviridae; Picornaviridae Infections - epidemiology; Picornaviridae Infections - veterinary; Picornaviridae Infections - virology; Picornaviruses; Polymerase chain reaction; Real time; Rectum; Seneca Valley virus; Senecavirus a; Shedding; Suckling behavior; Swine; Swine Diseases - epidemiology; Swine Diseases - virology; Thermal cycling; Tonsil; Vascular Diseases - epidemiology; Vascular Diseases - veterinary; Vascular Diseases - virology; Vesicular disease; Viremia; Virus diseases; Virus Shedding; Viruses; Weaning; United States; Brazil; United States > US; Shedding; Case report; Senecavirus a; Swine; Seneca Valley virus; Epidemiology; Vesicular disease
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-017-1172-7

The study highlights the shedding pattern of Senecavirus A (SVA) during an outbreak of vesicular disease in a sow farm from the South-central Minnesota, USA. In this study, 34 individual, mixed parity sows with clinical signs of vesicular lesions and 30 individual piglets from 15 individual litters from sows with vesicular lesions were conveniently selected for individual, longitudinal sampling. Serum, tonsil, rectal, and vesicular swabs were collected on day1 post outbreak, and then again at 1, 2, 3, 4, 6, and 9 weeks post outbreak. Samples were tested at the University of Minnesota Veterinary Diagnostic Laboratory for SVA via Real Time Polymerase Chain Reaction (RT-PCR) RESULTS: In sows, vesicular lesions had the highest concentration of SVA, but had the shortest duration of detection lasting only 2 weeks. Viremia was detected for 1 week post outbreak, and quickly declined thereafter. SVA was detected at approximately the same frequency for both tonsil and rectal swabs with the highest percentage of SVA positive samples detected in the first 6 weeks post outbreak. In suckling piglets, viremia quickly declined 1 week post outbreak and was prevalent in low levels during the first week after weaning (4 weeks post outbreak) and was also detected in piglets that were co-mingled from a SVA negative sow farm. Similar to sows, SVA detection on rectal and tonsil swabs in piglets lasted approximately 6 weeks post outbreak. The study illustrates the variation of SVA shedding patterns in different sample types over a 9 week period in sows and piglets, and suggests the potential for viral spread between piglets at weaning.