The First Genomic and Proteomic Characterization of a Deep-Sea Sulfate Reducer: Insights into the Piezophilic Lifestyle of Desulfovibrio piezophilus

• Published in: PloS one Vol. 8; no. 1; p. e55130
• Main Authors: Pradel, Nathalie, Ji, Boyang, Gimenez, Grégory, Talla, Emmanuel, Lenoble, Patricia, Garel, Marc, Tamburini, Christian, Fourquet, Patrick, Lebrun, Régine, Bertin, Philippe, Denis, Yann, Pophillat, Matthieu, Barbe, Valérie, Ollivier, Bernard, Dolla, Alain
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 2013; Public Library of Science (PLoS)
• Subjects: Adaptation; Adaptation, Physiological - genetics; Alanine; Amino acids; Amino Acids - metabolism; Arginine; Asparagine; Aspartic acid; Atmospheric Pressure; Bacteria; Bacteriology; Biochemistry, Molecular Biology; Biology; Comparative analysis; Deep sea; Desulfovibrio; Desulfovibrio - genetics; Desulfovibrio - metabolism; Desulfovibrio - physiology; E coli; Ecosystems; Energy Metabolism - genetics; Escherichia coli; Gene clusters; Genes; Genomes; Genomic islands; Genomics; Glutamic acid; Histidine; Hydrostatic Pressure; Life Sciences; Lifestyles; Lipids - biosynthesis; Lysine; Metabolism; Microbiology and Parasitology; Microorganisms; Multigene Family - genetics; Nucleotide sequence; Oceans and Seas; Oxidation-Reduction; Pressure; Proteins; Proteomics; Serine; Solutes; Species Specificity; Sulfate reduction; Sulfate-reducing bacteria; Sulfates; Sulfates - metabolism; Sulfur; Threonine; Tyrosine; Wood; France; Protein metabolism; Hydrostatic pressure; Biological transport; Outer membrane proteins; Extremophiles; Comparative genomics; Biosynthesis; Sulfates
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0055130

Desulfovibrio piezophilus strain C1TLV30(T) is a piezophilic anaerobe that was isolated from wood falls in the Mediterranean deep-sea. D. piezophilus represents a unique model for studying the adaptation of sulfate-reducing bacteria to hydrostatic pressure. Here, we report the 3.6 Mbp genome sequence of this piezophilic bacterium. An analysis of the genome revealed the presence of seven genomic islands as well as gene clusters that are most likely linked to life at a high hydrostatic pressure. Comparative genomics and differential proteomics identified the transport of solutes and amino acids as well as amino acid metabolism as major cellular processes for the adaptation of this bacterium to hydrostatic pressure. In addition, the proteome profiles showed that the abundance of key enzymes that are involved in sulfate reduction was dependent on hydrostatic pressure. A comparative analysis of orthologs from the non-piezophilic marine bacterium D. salexigens and D. piezophilus identified aspartic acid, glutamic acid, lysine, asparagine, serine and tyrosine as the amino acids preferentially replaced by arginine, histidine, alanine and threonine in the piezophilic strain. This work reveals the adaptation strategies developed by a sulfate reducer to a deep-sea lifestyle.