Sensing Size through Clustering in Non-Equilibrium Membranes and the Control of Membrane-Bound Enzymatic Reactions

• Published in: PloS one Vol. 10; no. 12; p. e0143470
• Main Authors: Vagne, Quentin, Turner, Matthew S, Sens, Pierre
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.12.2015; Public Library of Science (PLoS)
• Subjects: Biological Physics; Cell Membrane - chemistry; Cell Membrane - enzymology; Cell Membrane - metabolism; Cell Physiological Phenomena; Cluster Analysis; Clustering; Computer simulation; Confinement; Cytoskeleton; Enzymes; Equilibrium; Fluxes; Lipids; Membrane fluidity; Membrane Microdomains - metabolism; Membrane proteins; Membrane Proteins - chemistry; Membrane Proteins - metabolism; Membranes; Metabolism; Models, Biological; Models, Chemical; Organelles; Physics; Physiology; Plasma; Proteins; Recycling; Size distribution; Stochastic Processes; Stochasticity; France
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0143470

The formation of dynamical clusters of proteins is ubiquitous in cellular membranes and is in part regulated by the recycling of membrane components. We show, using stochastic simulations and analytic modeling, that the out-of-equilibrium cluster size distribution of membrane components undergoing continuous recycling is strongly influenced by lateral confinement. This result has significant implications for the clustering of plasma membrane proteins whose mobility is hindered by cytoskeletal "corrals" and for protein clustering in cellular organelles of limited size that generically support material fluxes. We show how the confinement size can be sensed through its effect on the size distribution of clusters of membrane heterogeneities and propose that this could be regulated to control the efficiency of membrane-bound reactions. To illustrate this, we study a chain of enzymatic reactions sensitive to membrane protein clustering. The reaction efficiency is found to be a non-monotonic function of the system size, and can be optimal for sizes comparable to those of cellular organelles.