Regional myocardial strain analysis via 2D speckle tracking echocardiography: validation with sonomicrometry and correlation with regional blood flow in the presence of graded coronary stenoses and dobutamine stress

• Published in: Cardiovascular ultrasound Vol. 18; no. 1; p. 2
• Main Authors: Stendahl, John C, Parajuli, Nripesh, Lu, Allen, Boutagy, Nabil E, Guerrera, Nicole, Alkhalil, Imran, Lin, Ben A, Staib, Lawrence H, O'Donnell, Matthew, Duncan, James S, Sinusas, Albert J
• Format: Journal Article
• Language: English
• Published: England BioMed Central 15.01.2020; BMC
• Subjects: Animals; Blood flow; Cardiovascular disease; Catheters; Circumferences; Computer programs; Coronary artery; Coronary Circulation - physiology; Coronary Stenosis - diagnosis; Coronary Stenosis - physiopathology; Coronary Vessels - diagnostic imaging; Coronary Vessels - physiopathology; Correlation analysis; Crystals; Data acquisition; Disease Models, Animal; Dobutamine stress echocardiography; Dogs; Echocardiography; Echocardiography, Stress - methods; Evaluation; Heart; Image registration; Ischemia; Laboratory animals; Low flow; Myocardial Contraction - physiology; Myocardial ischemia; Myocardial strain; Myocardium; Perfusion; Regional Blood Flow - physiology; Software; Sonomicrometry; Speckle tracking; Stress; Systole; Two dimensional analysis; Two dimensional models; Ultrasonic imaging; Veins & arteries; Viability; Dobutamine stress echocardiography; Speckle tracking; Myocardial strain; Perfusion; Sonomicrometry
• ISSN: 1476-7120; 1476-7120
• DOI: 10.1186/s12947-019-0183-x

Quantitative regional strain analysis by speckle tracking echocardiography (STE) may be particularly useful in the assessment of myocardial ischemia and viability, although reliable measurement of regional strain remains challenging, especially in the circumferential and radial directions. We present an acute canine model that integrates a complex sonomicrometer array with microsphere blood flow measurements to evaluate regional myocardial strain and flow in the setting of graded coronary stenoses and dobutamine stress. We apply this unique model to rigorously evaluate a commercial 2D STE software package and explore fundamental regional myocardial flow-function relationships. Sonomicrometers (16 crystals) were implanted in epicardial and endocardial pairs across the anterior myocardium of anesthetized open chest dogs (n = 7) to form three adjacent cubes representing the ischemic, border, and remote regions, as defined by their relative locations to a hydraulic occluder on the mid-left anterior descending coronary artery (LAD). Additional cardiac (n = 3) and extra-cardiac (n = 3) reference crystals were placed to define the cardiac axes and aid image registration. 2D short axis echocardiograms, sonometric data, and microsphere blood flow data were acquired at baseline and in the presence of mild and moderate LAD stenoses, both before and during low-dose dobutamine stress (5 μg/kg/min). Regional end-systolic 2D STE radial and circumferential strains were calculated with commercial software (EchoInsight) and compared to those determined by sonomicrometry and to microsphere blood flow measurements. Post-systolic indices (PSIs) were also calculated for radial and circumferential strains. Low-dose dobutamine augmented both strain and flow in the presence of mild and moderate stenoses. Regional 2D STE strains correlated moderately with strains assessed by sonomicrometry (R  = 0.56, p < 0.0001; R  = 0.55, p < 0.0001) and with regional flow quantities (R  = 0.61, R  = 0.63). Overall, correspondence between 2D STE and sonomicrometry was better in the circumferential direction (Bias ± 1.96 SD: - 1.0 ± 8.2% strain, p = 0.06) than the radial direction (5.7 ± 18.3%, p < 0.0001). Mean PSI values were greatest in low flow conditions and normalized with low-dose dobutamine. 2D STE identifies changes in regional end-systolic circumferential and radial strain produced by mild and moderate coronary stenoses and low-dose dobutamine stress. Regional 2D STE end-systolic strain measurements correlate modestly with regional sonomicrometer strain and microsphere flow measurements.