Comparing CT perfusion with oxygen partial pressure in a rabbit VX2 soft-tissue tumor model

• Published in: Journal of radiation research Vol. 55; no. 1; pp. 183 - 190
• Main Authors: Sun, Chang-Jin, Li, Chao, Lv, Hai-Bo, Zhao, Cong, Yu, Jin-Ming, Wang, Guang-Hui, Luo, Yun-Xiu, Li, Yan, Xiao, Mingyong, Yin, Jun, Lang, Jin-Yi
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.01.2014
• Subjects: Animals; Blood Flow Velocity; Blood volume; Brain; Brain cancer; Brain tumors; CAT scans; Construction; Correlation; Diagnostic imaging; Female; Male; Microelectrodes; Neovascularization, Pathologic - diagnostic imaging; Neovascularization, Pathologic - metabolism; Oximetry - methods; Oxygen; Oxygen - metabolism; Partial pressure; Perfusion Imaging - methods; Polyetherimides; Rabbits; Radiography; Reproducibility of Results; Sensitivity and Specificity; Soft Tissue Neoplasms - diagnostic imaging; Soft Tissue Neoplasms - metabolism; Technology; Tumors; hypoxia; perfusion computed tomography; rabbit model; oxygen partial pressure; VX2 tumor
• ISSN: 0449-3060; 1349-9157; 1349-9157
• DOI: 10.1093/jrr/rrt092

The aim of this study was to evaluate the oxygen partial pressure of the rabbit model of the VX2 tumor using a 64-slice perfusion CT and to compare the results with that obtained using the oxygen microelectrode method. Perfusion CT was performed for 45 successfully constructed rabbit models of a VX2 brain tumor. The perfusion values of the brain tumor region of interest, the blood volume (BV), the time to peak (TTP) and the peak enhancement intensity (PEI) were measured. The results were compared with the partial pressure of oxygen (PO2) of that region of interest obtained using the oxygen microelectrode method. The perfusion values of the brain tumor region of interest in 45 successfully constructed rabbit models of a VX2 brain tumor ranged from 1.3–127.0 (average, 21.1 ± 26.7 ml/min/ml); BV ranged from 1.2–53.5 ml/100g (average, 22.2 ± 13.7 ml/100g); PEI ranged from 8.7–124.6 HU (average, 43.5 ± 28.7 HU); and TTP ranged from 8.2–62.3 s (average, 38.8 ± 14.8 s). The PO2 in the corresponding region ranged from 0.14–47 mmHg (average, 16 ± 14.8 mmHg). The perfusion CT positively correlated with the tumor PO2, which can be used for evaluating the tumor hypoxia in clinical practice.