The immune mechanism of Mycoplasma hyopneumoniae 168 vaccine strain through dendritic cells

• Published in: BMC veterinary research Vol. 13; no. 1; p. 285
• Main Authors: Shen, Yumeng, Hu, Weiwei, Wei, Yanna, Feng, Zhixin, Yang, Qian
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 15.09.2017; BioMed Central; BMC
• Subjects: Analysis; Animals; Antigen-presenting cells; Antigens; Bacterial Vaccines - immunology; Bone marrow; Causes of; CD80 antigen; Cell proliferation; Cells, Cultured; Cytokines; Dendritic cells; Dendritic Cells - immunology; Drug resistance; Enzootic pneumonia; Epithelium; Exposure; Flow cytometry; Granulocytes; Hogs; Immune response; Infections; Interferon; Interleukin 10; Lymphocytes; Lymphocytes T; Macrophages - physiology; Mycoplasma; Mycoplasma hyopneumoniae - immunology; Pneumonia; Pneumonia of Swine, Mycoplasmal - microbiology; Pneumonia of Swine, Mycoplasmal - prevention & control; Respiratory tract; Specific Pathogen-Free Organisms; Suidae; Swine; T cell receptors; T cells; Vaccine Mhp-168 strain, Dendritic cells, Immune protection; Vaccines; Viral infections; United States > US; Vaccine Mhp-168 strain, Dendritic cells, Immune protection
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-017-1194-1

Mycoplasma hyopneumoniae (Mhp) causes porcine enzootic pneumonia, a disease that cause major economic losses in the pig industry. Dendritic cells (DCs), the most effective antigen-presenting cells, are widely distributed beneath respiratory epithelium, DCs uptake and present antigens to T cells, to initiate protective immune responses in different infections. In this study, we investigated the role of porcine DCs in vaccine Mhp-168 exposure. The antigen presenting ability of DCs were improved by vaccine Mhp-168 exposure. DCs could activate T-cell proliferation by up-regulating the antigen presenting molecule MHCII expression and co-stimulatory molecule CD80/86. However, the up-regulation of IL-10 and accompany with down-regulation of IFN-γ gene level may account for the limitation of attenuated Mhp-168 strain use as vaccine alone. These findings are benefit for exploring the protection mechanisms and the possible limitations of this attenuated Mhp-168 vaccine.