Single administration of 1-benzyl-1,2,3,4-tetrahydroisoquinoline increases the extracellular concentration of dopamine in rat striatum

• Published in: Neuroscience Vol. 160; no. 4; pp. 820 - 828
• Main Authors: Katagiri, N, Abe, K, Kitabatake, M, Utsunomiya, I, Horiguchi, Y, Hoshi, K, Taguchi, K
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 02.06.2009; Elsevier
• Subjects: 1-benzyl-1,2,3,4-tetrahydroisoquinoline; Animals; Biological and medical sciences; Corpus Striatum - drug effects; Corpus Striatum - metabolism; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; dopamine; Dopamine - metabolism; Dopamine - secretion; Dopamine Antagonists - pharmacology; Dopamine Plasma Membrane Transport Proteins - drug effects; Dopamine Plasma Membrane Transport Proteins - metabolism; Dopamine Uptake Inhibitors - pharmacology; Dose-Response Relationship, Drug; Drug Administration Schedule; Extracellular Fluid - drug effects; Extracellular Fluid - metabolism; Fundamental and applied biological sciences. Psychology; in vivo microdialysis; Male; Medical sciences; Microdialysis; Motor Activity - drug effects; Motor Activity - physiology; Neurology; Neurons - drug effects; Neurons - metabolism; Neurons - secretion; Oxidopamine - pharmacology; Parkinson's disease; Rats; Rats, Wistar; Sodium Channel Blockers - pharmacology; striatum; Substantia Nigra - drug effects; Substantia Nigra - metabolism; substantia nigra pars compacta; Sympatholytics; Tetrahydroisoquinolines - pharmacology; Tetrodotoxin - pharmacology; Vertebrates: nervous system and sense organs; 1,2,3,4-tetrahydroisoquinoline; Parkinson's disease; 1-BnTIQ; 1-benzyl-1,2,3,4-tetrahydroisoquinoline; 6-OHDA; 3,4-dehydroxyphenylacetic acid; 4-hydroxy-3-methoxyphenylacetic acid; AUC; striatum; 3-MT; TTX; 3-methoxytyramine; HPLC; DOPAC; SNc; area under the curve; substantia nigra pars compacta; high performance liquid chromatography; HVA; 6-hydroxydopamine; dopamine transporter; DAT; 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; MPTP; tetrodotoxin; dopamine; in vivo microdialysis; TIQ; Rat; Central nervous system; Parkinson disease; Encephalon; Degenerative disease; Isoquinoline(1,2,3,4-tétrahydro); Nervous system diseases; Dopamine; Rodentia; Basal ganglion; Corpus striatum; Catecholamine; Extracellular; Cerebral disorder; Vertebrata; Mammalia; Locus niger; Microdialysis; Animal; Central nervous system disease; Neurotransmitter; Extrapyramidal syndrome
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2009.03.009

Abstract We performed a combined neurochemical and behavioral study to determine the effects of 1-benzyl-1,2,3,4-tetrahydroisoquinoline (1-BnTIQ) on the extracellular dopamine concentrations in the striatum. Single dose administration of 1-BnTIQ (20, 40, and 80 mg/kg i.p.) increased striatal dopamine extracellular levels in a dose-dependent manner when an in vivo microdialysis technique was used to assess dopamine levels in the striatum of rats. Enhancement of striatal dopamine levels by systemic administration of a single dose of 1-BnTIQ was suppressed by perfusion of tetrodotoxin and a calcium ion-free solution into the striatum. This 1-BnTIQ-induced increase in extracellular dopamine concentration was also inhibited by pre-treatment with a dopamine uptake inhibitor, GBR12909 (1-(2-[bis(4-Fluorophenyl)-4-(3-phenylpropyl)piperazine dihydrochloride). Local application of 1-BnTIQ into the striatum via a dialysis probe failed to enhance the extracellular concentration of dopamine. However, microinjection of 1-BnTIQ into the substantia nigra pars compacta increased the extracellular dopamine levels in the striatum. Locomotor activity was increased by systemic administration of a single dose of 1-BnTIQ in a dose-dependent manner. This 1-BnTIQ-induced locomotor activity was attenuated by pre-treatment with SCH23390 (R(+)-7-Chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine hydrochlodride) and raclopride, D1 and D2 dopaminergic receptor antagonists, respectively. Moreover, 1-BnTIQ induced ipsilateral rotational behavior in 6-hydroxydopamine–lesioned rats. These results suggest that systemic administration of a single dose of 1-BnTIQ increases striatal extracellular dopamine concentration through activation of dopaminergic nigra striatal neurons via the dopamine transporter.