Pharmacological or genetic targeting of Transient Receptor Potential (TRP) channels can disrupt the planarian escape response

• Published in: PloS one Vol. 14; no. 12; p. e0226104
• Main Authors: Sabry, Ziad, Ho, Alicia, Ireland, Danielle, Rabeler, Christina, Cochet-Escartin, Olivier, Collins, Eva-Maria S
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05.12.2019; Public Library of Science (PLoS)
• Subjects: Allyl isothiocyanate; Amputation; Anandamide; Animals; Antagonists; Biology and Life Sciences; Biomechanics; Cannabinoids; Capsaicin; Channels; Developmental biology; Escape behavior; Escape Reaction - drug effects; Gene expression; Gliding; Heat; Hydrogen; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Invertebrates; Isothiocyanate; Isothiocyanates - pharmacology; Latency; Life Sciences; Locomotion; Mammals; Medicine and Health Sciences; Nociception - drug effects; Organic chemistry; Pain; Pain perception; Physical Sciences; Physics; Planarians; Regulatory mechanisms (biology); Research and Analysis Methods; RNA-mediated interference; Stimuli; Transient Receptor Potential Channels - agonists; Transient Receptor Potential Channels - genetics; Transient receptor potential proteins; Turbellaria; La Jolla California; California; United States > US; Pennsylvania
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0226104

In response to noxious stimuli, planarians cease their typical ciliary gliding and exhibit an oscillatory type of locomotion called scrunching. We have previously characterized the biomechanics of scrunching and shown that it is induced by specific stimuli, such as amputation, noxious heat, and extreme pH. Because these specific inducers are known to activate Transient Receptor Potential (TRP) channels in other systems, we hypothesized that TRP channels control scrunching. We found that chemicals known to activate TRPA1 (allyl isothiocyanate (AITC) and hydrogen peroxide) and TRPV (capsaicin and anandamide) in other systems induce scrunching in the planarian species Dugesia japonica and, except for anandamide, in Schmidtea mediterranea. To confirm that these responses were specific to either TRPA1 or TRPV, respectively, we tried to block scrunching using selective TRPA1 or TRPV antagonists and RNA interference (RNAi) mediated knockdown. Unexpectedly, co-treatment with a mammalian TRPA1 antagonist, HC-030031, enhanced AITC-induced scrunching by decreasing the latency time, suggesting an agonistic relationship in planarians. We further confirmed that TRPA1 in both planarian species is necessary for AITC-induced scrunching using RNAi. Conversely, while co-treatment of a mammalian TRPV antagonist, SB-366791, also enhanced capsaicin-induced reactions in D. japonica, combined knockdown of two previously identified D. japonica TRPV genes (DjTRPVa and DjTRPVb) did not inhibit capsaicin-induced scrunching. RNAi of DjTRPVa/DjTRPVb attenuated scrunching induced by the endocannabinoid and TRPV agonist, anandamide. Overall, our results show that although scrunching induction can involve different initial pathways for sensing stimuli, this behavior's signature dynamical features are independent of the inducer, implying that scrunching is a stereotypical planarian escape behavior in response to various noxious stimuli that converge on a single downstream pathway. Understanding which aspects of nociception are conserved or not across different organisms can provide insight into the underlying regulatory mechanisms to better understand pain sensation.