Inhibitory effect of tyrphostin 47 on Shiga toxin-induced cell death

• Published in: European Journal of Pharmacology Vol. 546; no. 1; pp. 36 - 39
• Main Authors: Ikeda, Masahiro, Gunji, Yasuhiro, Sonoda, Hiroko, Oshikawa, Sayaka, Shimono, Mariko, Horie, Atsuko, Ito, Katsuaki, Yamasaki, Shinji
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 28.09.2006; Elsevier BV; Elsevier
• Subjects: Animals; Biological and medical sciences; Cell Death; Cell Death - drug effects; Cercopithecus aethiops; Chlorocebus aethiops; Escherichia coli; MAP Kinase Signaling System; MAP Kinase Signaling System - drug effects; Medical sciences; p38 MAPK; p38 Mitogen-Activated Protein Kinases; p38 Mitogen-Activated Protein Kinases - metabolism; Pharmacology. Drug treatments; Phosphorylation; Protein Kinase Inhibitors; Protein Kinase Inhibitors - pharmacology; Shiga Toxin; Shiga Toxin - toxicity; Tyrosine kinase; Tyrosine kinase inhibitor; Tyrphostin 47; Tyrphostins; Tyrphostins - pharmacology; Vero Cells; Tyrosine kinase; Shiga toxin; Tyrosine kinase inhibitor; Tyrphostin 47; p38 MAPK; Toxin; Enzyme; Cell death; Transferases; Enzyme inhibitor; Mitogen-activated protein kinase; Protein-tyrosine kinase
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/j.ejphar.2006.07.014

The inhibitory effects of tyrosine kinase inhibitors including tyrphostins 25, 47 and 51 on Shiga toxin 1-induced cell death and p38 mitogen-activated protein kinase (p38 MAPK) phosphorylation were examined in Vero cells. Tyrphostin 47 significantly inhibited Shiga toxin 1-induced cell death and p38 MAPK phosphorylation. In contrast, tyrphostins 25 and 51 had no significant effect on the Shiga toxin 1-induced responses. These data indicate that Shiga toxin 1-induced cell injury occurs through a pathway sensitive to tyrphostin 47, and the target molecule for tyrphostin 47 opens up new opportunities for pharmacological intervention against Shiga toxin-producing Escherichia coli infectious diseases.