Neuroblastoma patient-derived orthotopic xenografts reflect the microenvironmental hallmarks of aggressive patient tumours

• Published in: Cancer letters Vol. 375; no. 2; pp. 384 - 389
• Main Authors: Braekeveldt, Noémie, Wigerup, Caroline, Tadeo, Irene, Beckman, Siv, Sandén, Caroline, Jönsson, Jimmie, Erjefält, Jonas S, Berbegall, Ana P, Börjesson, Anna, Backman, Torbjörn, Øra, Ingrid, Navarro, Samuel, Noguera, Rosa, Gisselsson, David, Påhlman, Sven, Bexell, Daniel
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.06.2016; Elsevier Limited
• Subjects: Animals; Blood Vessels - pathology; Cancer; Cancer and Oncology; Cancer och onkologi; Clinical Medicine; Disease Models, Animal; Ethics; Genotype; Hematology, Oncology and Palliative Medicine; Humans; Immune system; Immunotherapy; Klinisk medicin; Laboratory animals; Medical and Health Sciences; Medical prognosis; Medical research; Medicin och hälsovetenskap; Metastasis; Mice; Neovascularization, Pathologic - genetics; Neovascularization, Pathologic - pathology; Neuroblastoma; Neuroblastoma - genetics; Neuroblastoma - pathology; Paediatric cancer; Patient-derived xenograft (PDX); Patients; Polymorphism, Single Nucleotide; Quantitative analysis; Tumor Microenvironment - genetics; Tumors; Tumour microenvironment; Tumour stroma; Xenograft Model Antitumor Assays; α-smooth muscle actin; huCD31; IHC; NCAM; single nucleotide polymorphism; neural cell adhesion molecule; tumour-associated macrophages; Neuroblastoma; Metastasis; human-specific CD31; CAFs; SNP; TAMs; Paediatric cancer; NOD/SCID/gamma(c)(null); Patient-derived xenograft (PDX); immunohistochemistry; LYVE-1; mouse CD34; moCD34; Tumour stroma; NSG; lymphatic vessel endothelial hyaluronan receptor 1; PDXs; TME; Tumour microenvironment; ECM; patient-derived xenografts; cancer-associated fibroblasts; αSMA; extracellular matrix
• ISSN: 0304-3835; 1872-7980; 1872-7980
• DOI: 10.1016/j.canlet.2016.02.046

Highlights • Patient-derived tumour endothelial cells co-engraft in neuroblastoma PDXs. • PDXs contain fibroblasts, pericytes, endothelium, immune cells, and lymphatic vessels. • PDXs resemble the ECM features of aggressive neuroblastoma tumours. • PDXs faithfully reflect the stromal hallmarks of aggressive patient tumours. • Neuroblastoma orthotopic PDXs are clinically relevant models for drug testing.