Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus

• Published in: The journal of microbiology Vol. 51; no. 1; pp. 130 - 135
• Main Authors: Shim, Byoung-Shik, Choi, Jung-ah, Song, Ho-Hyun, Park, Sung-Moo, Cheon, In Su, Jang, Ji-Eun, Woo, Sun Je, Cho, Chung Hwan, Song, Min-Suk, Kim, Hyemi, Song, Kyung Joo, Lee, Jae Myun, Kim, Suhng Wook, Song, Dae Sub, Choi, Young Ki, Kim, Jae-Ouk, Nguyen, Huan Huu, Kim, Dong Wook, Bahk, Young Yil, Yun, Cheol-Heui, Song, Man Ki
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.02.2013; The Microbiological Society of Korea; Springer Nature B.V; 한국미생물학회
• Subjects: Adjuvants, Immunologic; Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - genetics; administration & dosage; Administration, Sublingual; Agricultural biotechnology; analysis; Animals; Antibodies; Antibodies, Neutralizing; Antibodies, Neutralizing - analysis; Antibodies, Neutralizing - blood; Antibodies, Viral; Antibodies, Viral - analysis; Antibodies, Viral - blood; Bacteria; Biomedical and Life Sciences; blood; cholera toxin; Cholera Toxin - administration & dosage; Cholera Toxin - genetics; Disease Models, Animal; Enzyme-Linked Immunospot Assay; genetics; Hemagglutination Inhibition Tests; Hemagglutinin Glycoproteins, Influenza Virus; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinin Glycoproteins, Influenza Virus - immunology; hemagglutinins; humans; Immunity, Mucosal; Immunization; immunology; Infections; influenza; Influenza A virus; Influenza A Virus, H1N1 Subtype; Influenza A Virus, H1N1 Subtype - genetics; Influenza A Virus, H1N1 Subtype - immunology; Influenza Vaccines; Influenza Vaccines - administration & dosage; Influenza Vaccines - genetics; Influenza Vaccines - immunology; Influenza virus; intramuscular injection; Leukocytes, Mononuclear; Leukocytes, Mononuclear - immunology; Life Sciences; Lung; Lung - virology; Mice; Mice, Inbred BALB C; Microbiology; morbidity; mortality; mucosal immunity; Nervous system; neutralization; oral administration; Orthomyxoviridae Infections; Orthomyxoviridae Infections - prevention & control; Outbreaks; pandemic; Pandemics; Pathogens; Pharmacy; prevention & control; Proteins; Public health; recombinant proteins; Research centers; risk; Serum; Serum - immunology; Swine flu; Toxins; vaccination; Vaccines; Vaccines, Synthetic; Vaccines, Synthetic - administration & dosage; Vaccines, Synthetic - genetics; Vaccines, Synthetic - immunology; Viral Load; Virology; Viruses; Waterborne diseases; 생물학; United States > US; South Korea; mucosal immune response; non-glycosylation; pandemic; Hemagglutinin; sublingual
• ISSN: 1225-8873; 1976-3794; 1976-3794
• DOI: 10.1007/s12275-013-2399-z

Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a well-established bacterial expression system and administered it into mice via sublingual (s.l.) route. We found that s.l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i.m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s.l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.