Live nanoscopic to mesoscopic topography reconstruction with an optical microscope for chemical and biological samples

Macroscopic properties of physical and biological processes like friction, wetting, and adhesion or cell migration are controlled by interfacial properties at the nanoscopic scale. In an attempt to bridge simultaneously investigations at different scales, we demonstrate here how optical microscopy i...

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Published inPloS one Vol. 13; no. 12; p. e0207881
Main Authors Theodoly, Olivier, Garcia-Seyda, Nicolas, Bedu, Fréderic, Luo, Xuan, Gabriele, Sylvain, Mignot, Tâm, Giermanska, Joanna, Chapel, Jean-Paul, Métivier, Mélinda, Valignat, Marie-Pierre
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 12.12.2018
Public Library of Science (PLoS)
Subjects
Adsorption
Biological activity
Biological properties
Biological samples
Calibration
Cell adhesion & migration
Cell migration
Condensed Matter
Ellipsometry
Engineering and Technology
Friction
Interfaces
Interfacial properties
Life Sciences
Materials Science
Methods
Microfluidic devices
Microfluidics
Microscopy
Microscopy - methods
Motility
Optical microscopy
Optical properties
Optics
Organic chemistry
Phenotypes
Physical Sciences
Physics
Polymers
Proteins
Research and Analysis Methods
Surface Properties
Thin films
Tomography, X-Ray Computed - methods
Topography
Wettability
France
United States > US
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Summary:Macroscopic properties of physical and biological processes like friction, wetting, and adhesion or cell migration are controlled by interfacial properties at the nanoscopic scale. In an attempt to bridge simultaneously investigations at different scales, we demonstrate here how optical microscopy in Wet-Surface Ellipsometric Enhanced Contrast (Wet-SEEC) mode offers imaging and measurement of thin films at solid/liquid interfaces in the range 1-500 nm with lateral optical resolution. A live, label-free and noninvasive methodology integrated with microfluidic devices allowed here characterization of polymers and proteins patterns together with corresponding phenotypes of living cells.
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Competing Interests: We have the following interests: This work was supported by the Nanolane company (Le Mans), Solvay company (Bordeaux), Alveole company (Paris). MM was employed by Nanolane. There are no patents, products in development or marketed products to declare. This does not alter our adherence to all the PLOS ONE policies on sharing data and materials.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0207881