Ny-1 and Ny-2 genes conferring hypersensitive response to potato virus Y (PVY) in cultivated potatoes: mapping and marker-assisted selection validation for PVY resistance in potato breeding

• Published in: Molecular breeding Vol. 34; no. 1; pp. 267 - 271
• Main Authors: Szajko, Katarzyna, Strzelczyk-Żyta, Danuta, Marczewski, Waldemar
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.06.2014; Springer Netherlands; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biotechnology; Breeding; Chromosome 9; Chromosomes; Cultivars; Disease resistance; Gene mapping; genes; High temperature; Hypersensitive response; leaves; Life Sciences; Mapping; Marker-assisted selection; Markers; Molecular biology; Pathogens; Plant biology; Plant breeding; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant reproduction; Plant Sciences; Potato virus Y; Potatoes; Short Communication; Solanum tuberosum; temperature; Temperature dependence; Vegetables; Viruses; PVY; Mapping; Marker-assisted selection; Potato cultivars; Hypersensitivity
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-014-0024-4

Potato virus Y (PVY) is one of the most important viruses affecting potato (Solanum tuberosum) production. In this study, a novel hypersensitive response (HR) gene, Ny-2, conferring resistance to PVY was mapped on potato chromosome XI in cultivar Romula. In cultivars Albatros and Sekwana, the Ny-1 gene was mapped on chromosome IX. In cv. Romula, the local lesions appeared in leaves inoculated with the PVYᴺ⁻ᵂⁱ isolate at 20 and 28 °C; PVY systemic infections were only occasionally observed at the higher temperature. In cvs. Albatros and Sekwana, expression of the necrotic reaction to virus infection was temperature-dependent. PVYᴺ⁻ᵂⁱ was localized at 20 °C; at 28 °C, the systemic, symptomless infection was observed. We developed the B11.6₁₆₀₀ marker co-segregating with Ny-2 and the S1d11 marker specific for the Ny-1 gene. Fifty potato cultivars were tested with markers B11.6 and S1d11 and marker SC895 linked to the Ny-1 gene in cv. Rywal. These results indicated the utility of these markers for marker-assisted selection of HR-like PVY resistance in potato breeding programs.