Milk-Compositional Study of Metabolites and Pathogens in the Milk of Bovine Animals Affected with Subclinical Mastitis

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 23; p. 8631
• Main Authors: Ali, Aarif, Mir, Manzoor Ur Rahman, Ganie, Showkat Ahmad, Mushtaq, Saima, Bukhari, Sarah I, Alshehri, Sultan, Rashid, Shahzada Mudasir, Mir, Tahir Maqbool, Rehman, Muneeb U
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.12.2022; MDPI
• Subjects: Analysis; Animals; antibiotic sensitivity test; Antibiotics; Bacteria; Biosynthesis; Cattle; Cell Count; Cow's milk; Dairy cattle; Doxycycline; Drug resistance; Electrical conductivity; Electrical resistivity; Escherichia coli; Fatty acids; Female; Fever; Gas chromatography; Gentamicin; Hexanoic acid; Humans; Infections; Lauric acid; Libraries; Lipids; Mass spectrometry; Mass spectroscopy; Mastitis; Mastitis, Bovine; Metabolic pathways; Metabolism; Metabolites; metabolome; microbiome; Milk; Milk - chemistry; Octanoic acid; Palmitic acid; Pathogenic microorganisms; Pathogens; Saturated fatty acids; Sensitivity; Staphylococcal Infections - drug therapy; Staphylococcal Infections - veterinary; Staphylococcus aureus; subclinical mastitis; India; milk; antibiotic sensitivity test; microbiome; metabolome; subclinical mastitis
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27238631

Bovine milk is an important food component in the human diet due to its nutrient-rich metabolites. However, bovine subclinical mastitis alters the composition and quality of milk. In present study, California mastitis testing, somatic cell count, pH, and electrical conductivity were used as confirmatory tests to detect subclinical mastitis. The primary goal was to study metabolome and identify major pathogens in cows with subclinical mastitis. In this study, 29 metabolites were detected in milk using gas chromatography−mass spectrometry. Volatile acidic compounds, such as hexanoic acid, hexadecanoic acid, lauric acid, octanoic acid, n-decanoic acid, tricosanoic acid, tetradecanoic acid, and hypogeic acid were found in milk samples, and these impart good flavor to the milk. Metaboanalyst tool was used for metabolic pathway analysis and principal component estimation. In this study, EC and pH values in milk were significantly increased (p < 0.0001), whereas fat (p < 0.04) and protein (p < 0.0002) significantly decreased in animals with subclinical mastitis in comparison to healthy animals. Staphylococcus aureus was the predominant pathogen found (n = 54), followed by Escherichia coli (n = 30). Furthermore, antibiotic sensitivity revealed that Staphylococcus aureus was more sensitive to gentamicin (79.6%), whereas Escherichia coli showed more sensitivity to doxycycline hydrochloride (80%).