The cytokine profile of women with severe anxiety and depression during pregnancy

• Published in: BMC psychiatry Vol. 19; no. 1; p. 104
• Main Authors: Leff Gelman, P, Mancilla-Herrera, I, Flores-Ramos, M, Saravia Takashima, M F, Cruz Coronel, F M, Cruz Fuentes, C, Pérez Molina, A, Hernández-Ruiz, J, Silva-Aguilera, F S, Farfan-Labonne, B, Chinchilla-Ochoa, D, Garza Morales, S, Camacho-Arroyo, I
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 03.04.2019; BioMed Central; BMC
• Subjects: Adult; Analysis; Anxiety; Anxiety - immunology; Anxiety Disorders; Biomarkers; Case-Control Studies; Chemokines; Comorbidity; Correlation analysis; Cytokines; Cytokines - blood; Data processing; Demographic aspects; Depression; Depression (Mood disorder); Depression - immunology; Female; Genetic aspects; Health care; Helper cells; Hormones; Humans; Immune system; Inflammation; Interleukin 10; Interleukin 13; Interleukin 2; Interleukin 22; Interleukin 4; Interleukin 6; Interleukin 9; Interleukin-10 - blood; Interleukin-17 - blood; Interleukins; Lymphocytes T; Mental depression; Obstetrics; Physiological aspects; Postpartum period; Pregnancy; Pregnancy Complications - immunology; Pregnant Women; Premature birth; Psychiatry; Psychological aspects; Stress; Tumor Necrosis Factor-alpha - blood; Tumor necrosis factor-α; Women; Womens health; Young Adult; γ-Interferon; Mexico; Pregnancy; Depression; Anxiety; Inflammation; Cytokines
• ISSN: 1471-244X; 1471-244X
• DOI: 10.1186/s12888-019-2087-6

Controversial findings regarding the association between pro-inflammatory cytokines and depression have been reported in pregnant subjects. Scarce data about anxiety and its relationships with cytokines are available in pregnant women. To understand the association between anxiety and cytokines during pregnancy, we conducted the present study in women with or without depression. Women exhibiting severe depression (SD) and severe anxiety (SA) during the 3rd trimester of pregnancy (n = 139) and control subjects exhibiting neither depression nor anxiety (n = 40) were assessed through the Hamilton Depression Rating Scale (HDRS) and the Hamilton Anxiety Rating Scale (HARS). Serum cytokines were measured by a multiplex bead-based assay. Correlation tests were used to analyze the data and comparisons between groups were performed. A general linear model of analysis of variance was constructed using the group as a dependent variable, interleukin concentrations as independent variables, and HDRS/HARS scores and gestational weeks as covariables. The highest levels of Th1- (IL-6, TNF-α, IL-2, IFN-γ), Th17- (IL-17A, IL-22), and Th2- (IL-9, IL-10, and IL-13) related cytokines were observed in women with SD + SA. The SA group showed higher concentrations of Th1- (IL-6, TNF-α, IL-2, IFN-γ) and Th2- (IL-4, and IL-10) related cytokines than the controls. Positive correlations were found between HDRS and IL-2, IL-6, and TNF-α in the SA group (p < 0.03), and between HDRS and Th1- (IL-2, IL-6, TNF-α), Th2- (IL-9, IL-10, IL-13) and Th17- (IL-17A) cytokines (p < 0.05) in the SD + SA group. After controlling the correlation analysis by gestational weeks, the correlations that remained significant were: HDRS and IL-2, IL-6, IL-9, and IL-17A in the SD + SA group (p < 0.03). HARS scores correlated with IL-17A in the SA group and with IL-17A, IL-17F, and IL-2 in the SD + SA group (p < 0.02). The linear model of analysis of variance showed that HDRS and HARS scores influenced cytokine concentrations; only IL-6 and TNF-α could be explained by the group. We found that the cytokine profiles differ when comparing pregnant subjects exhibiting SA with comorbid SD against those showing only SA without depression.