Molecular Phylogeny and Taxonomy of Two Novel Brackish Water Hypotrich Ciliates, with the Establishment of a New Genus, Antiokeronopsis gen. n. (Ciliophora, Hypotrichia)

• Published in: The Journal of eukaryotic microbiology Vol. 61; no. 5; pp. 449 - 462
• Main Authors: Fan, Yangbo, Pan, Ying, Huang, Jie, Lin, Xiaofeng, Hu, Xiaozhong, Warren, Alan
• Format: Journal Article
• Language: English
• Published: United States BioOne 01.09.2014; Blackwell Publishing Ltd
• Subjects: Anteholosticha; Antiokeronopsis gen. n; Brackish; brackish water; China; Ciliophora; coasts; color; cortical granules; DNA, Protozoan - genetics; DNA, Ribosomal - genetics; Hypotrichida - classification; Hypotrichida - genetics; Hypotrichida - growth & development; Hypotrichida - isolation & purification; Molecular Sequence Data; Morphogenesis; new genus; nucleotide sequences; Phylogeny; ribosomal RNA; Seawater - parasitology; SSU rDNA; Urostylids; South China Sea; China; ISEW, South China Sea; ISEW, China, People's Rep., Guangdong Prov., Shenzhen; Anteholosticha; SSU rDNA; morphogenesis; Urostylids; Antiokeronopsis gen. n
• ISSN: 1066-5234; 1550-7408
• DOI: 10.1111/jeu.12125

Two novel brackish water urostyloid ciliates, Anteholosticha paramanca sp. n. and Antiokeronopsis flava gen. n., sp. n., isolated from the Shenzhen Mangrove Nature Protection Area on the coast of the South China Sea, were investigated using live observation and protargol impregnation techniques. Anteholosticha paramanca sp. n. is characterized by its spherical yellowish cortical granules arranged in lines, shortened midventral complex and three transverse cirri. Morphogenesis is similar to that in Anteholosticha manca. The new genus Antiokeronopsis is diagnosed by having a continuous adoral zone of membranelles, frontal cirri arranged in a bicorona, midventral complex composed of midventral pairs only, one marginal cirral row on each side, the presence of frontoterminal and transverse cirri, and the lack of buccal and caudal cirri. The type species A. flava sp. n. is characterized by its elongated body shape, brown to yellowish body color and two types of cortical granules. Small subunit ribosomal RNA gene sequence data justify the classification of both species. Phylogenetic analyses indicate that A. paramanca clusters with Bakuella subtropica within a clade that includes two other Anteholosticha species, while Antiokeronopsis groups within the core urostylids and is most closely related to the well‐known genera Pseudokeronopsis and Uroleptopsis.