Analysis of Gastric Microbiota by Pyrosequencing: Minor Role of Bacteria Other Than Helicobacter pylori in the Gastric Carcinogenesis
Background Little is known about the role of gastric microbiota except for Helicobacter pylori (HP) in human health and disease. We compared the differences of human gastric microbiota according to gastric cancer or control and HP infection status and assessed the role of bacteria other than HP. Met...
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Published in | Helicobacter (Cambridge, Mass.) Vol. 21; no. 5; pp. 364 - 374 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Blackwell Publishing Ltd
01.10.2016
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Background
Little is known about the role of gastric microbiota except for Helicobacter pylori (HP) in human health and disease. We compared the differences of human gastric microbiota according to gastric cancer or control and HP infection status and assessed the role of bacteria other than HP.
Methods
Gastric microbiota of 63 antral mucosal and 18 corpus mucosal samples were analyzed by bar‐coded 454 pyrosequencing of the 16S rRNA gene. Antral samples were divided into four subgroups based on HP positivity in pyrosequencing and the presence of cancer. The analysis was focused on bacteria other than HP, especially nitrosating or nitrate‐reducing bacteria (NB). The changes of NB in antral mucosa of 16 subjects were followed up.
Results
The number of NB other than HP (non‐HP‐NB) was two times higher in the cancer groups than in the control groups, but it did not reach statistical significance. The number of non‐HP‐NB tends to increase over time, but this phenomenon was prevented by HP eradication in the HP‐positive control group, but not in the HP‐positive cancer group.
Conclusion
We could not find the significant role of bacteria other than HP in the gastric carcinogenesis. |
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Bibliography: | ark:/67375/WNG-5S94LHD1-8 National Research Foundation (NRF) of Korea ArticleID:HEL12293 Korean government (MSIP) - No. 2011-0030001 istex:02F6351B8C12B283E64D3841114ECB537160074C Figure S1. The mean proportions of phylum in the subgroupsFigure S2. The proportion of HP and genus Streptococcus according to the degree of intestinal metaplasia (IM)Table S1. Baseline characteristics of clinical and pyrosequencing results of gastric corpus samples Table S2. The species of nitrosating bacteria and nitrate reducing bacteria ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1083-4389 1523-5378 |
DOI: | 10.1111/hel.12293 |