体外大鼠肠道菌群代谢脱氧雪腐镰刀菌烯醇的研究

运用超高效液相色谱串联四级杆/飞行时间质谱（UPLC-Q/TOF-MS）识别和确定脱氧雪腐镰刀菌烯醇（Deoxynialenol,DON）在大鼠肠道菌群体外代谢模型中的代谢产物,同时运用高效液相色谱串联三重四级杆质谱定量分析DON含量变化,比较各段肠管中厌氧菌群的代谢能力。结果表明,DON不能被大鼠小肠菌群代谢,而能被其大肠菌群代谢为脱环氧代谢产物脱环氧-脱氧雪腐镰刀菌烯醇（Deepoxy-deoxynialenol,DOM）。定量测定结果显示,大肠中盲肠菌群代谢能力最强。...

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Published in分析化学 Vol. 40; no. 1; pp. 119 - 123
Main Author 冯培生 沈建忠 吴海霞 李林霞 戴燕玉 刘凯丽 王莹 张素霞
Format Journal Article
LanguageChinese
Published 中国农业大学动物医学院,北京,100193 2012
Subjects
体外代谢
大鼠
肠道菌
脱氧雪腐镰刀菌烯醇
超高效液相色谱串联四级杆/飞行时间质谱
大鼠
体外代谢
肠道菌
脱氧雪腐镰刀菌烯醇
超高效液相色谱串联四级杆/飞行时间质谱
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ISSN0253-3820
DOI10.3724/SP.J.1096.2012.10208

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Summary:运用超高效液相色谱串联四级杆/飞行时间质谱（UPLC-Q/TOF-MS）识别和确定脱氧雪腐镰刀菌烯醇（Deoxynialenol,DON）在大鼠肠道菌群体外代谢模型中的代谢产物,同时运用高效液相色谱串联三重四级杆质谱定量分析DON含量变化,比较各段肠管中厌氧菌群的代谢能力。结果表明,DON不能被大鼠小肠菌群代谢,而能被其大肠菌群代谢为脱环氧代谢产物脱环氧-脱氧雪腐镰刀菌烯醇（Deepoxy-deoxynialenol,DOM）。定量测定结果显示,大肠中盲肠菌群代谢能力最强。
Bibliography:Transformation in vitro; Intestinal microflora; Rats; Deoxynivalenol; Ultra performance liquid chromatography/quadrupole time of flight tandem mass spectrometry
22-1125/O6
Deoxynivalenol（DON） is a prevalent trichothecene contaminant,which is harmful to human and animal health.In this study,we identified the metabolites of DON produced by intestinal microflora of rats using ultra performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry（UPLC-Q/TOF-MS）.By quantitative analysis of DON using triple quadrupole mass spectrometer,we found that the transformation capability of caecum was much stronger than that of colon and rectum,and no metabolite was found in the microflora culture media of small intestine after 24 h incubation at 37 ℃ in anaerobic condition.In microflora culture media of large intestine,only de-epoxy-DON（DOM） was found,and transformation capability of caecum was much stronger than other sections of large intestine.
FENG Pei-Sheng,SHEN Jian-Zhong,WU Hai-Xia,LI Lin-Xia, DAI Y
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2012.10208