Cell Culture-Based Assessment of Toxicity and Therapeutics of Phytochemical Antioxidants

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 3; p. 1087
• Main Authors: Asuzu, Peace C, Trompeter, Nicholas S, Cooper, Carlton R, Besong, Samuel A, Aryee, Alberta N A
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06.02.2022; MDPI
• Subjects: Animals; antioxidant activity; Antioxidants; Antioxidants - therapeutic use; Antioxidants - toxicity; Apoptosis; Biocompatibility; bioprinting; Cancer therapies; Cell culture; Cell growth; cell lines; Cytotoxicity; Herbal medicine; Humans; Kinases; medicinal plants; Metabolites; Natural products; Oxidative Stress; Phenolic compounds; Phenols; Phytochemicals; Phytochemicals - therapeutic use; Phytochemicals - toxicity; Plant extracts; Plant Extracts - therapeutic use; Plant Extracts - toxicity; Plants; Review; Toxicity Tests; apoptosis; medicinal plants; cell lines; antioxidant activity; bioprinting; cytotoxicity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27031087

Plant-derived natural products are significant resources for drug discovery and development including appreciable potentials in preventing and managing oxidative stress, making them promising candidates in cancer and other disease therapeutics. Their effects have been linked to phytochemicals such as phenolic compounds and their antioxidant activities. The abundance and complexity of these bio-constituents highlight the need for well-defined in vitro characterization and quantification of the plant extracts/preparations that can translate to in vivo effects and hopefully to clinical use. This review article seeks to provide relevant information about the applicability of cell-based assays in assessing anti-cytotoxicity of phytochemicals considering several traditional and current methods.