Phenotype and Function of CD209+ Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages

• Published in: PloS one Vol. 11; no. 10; p. e0165247
• Main Authors: Park, Kun Taek, ElNaggar, Mahmoud M, Abdellrazeq, Gaber S, Bannantine, John P, Mack, Victoria, Fry, Lindsay M, Davis, William C
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.10.2016; Public Library of Science (PLoS)
• Subjects: Adaptive immunity; Animal diseases; Animal species; Animals; Antibodies, Monoclonal - immunology; Antigens; Antigens, CD - metabolism; Artiodactyla; Artiodactyla - immunology; Artiodactyla - metabolism; Biology and life sciences; Blood; Cattle; CD4 antigen; CD8 antigen; Cdc2 protein; Cell Adhesion Molecules - immunology; Cell Adhesion Molecules - metabolism; Cell Differentiation; Cells, Cultured; Dendritic cells; Dendritic Cells - cytology; Dendritic Cells - metabolism; Dendritic structure; Divergence; Flow cytometry; Functional analysis; Genotype & phenotype; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology; Identification; Immunity; Interleukin-4 - pharmacology; Lectins, C-Type - immunology; Lectins, C-Type - metabolism; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - metabolism; Lymphocytes; Lymphocytes T; Macrophages; Macrophages - cytology; Macrophages - metabolism; Male; Medicine and health sciences; Monoclonal antibodies; Monocytes; Monocytes - cytology; Monocytes - drug effects; Mononuclear phagocyte system; Mycobacterium avium; Ontogeny; Pathology; Phenotype; Phenotypes; Phenotyping; Pigs; Priming; Receptors, Cell Surface - immunology; Receptors, Cell Surface - metabolism; Respiratory syncytial virus; Ruminantia; Studies; Swine; T cell receptors; Veterinary medicine; United States > US; Egypt
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0165247

Phylogenic comparisons of the mononuclear phagocyte system (MPS) of humans and mice demonstrate phenotypic divergence of dendritic cell (DC) subsets that play similar roles in innate and adaptive immunity. Although differing in phenotype, DC can be classified into four groups according to ontogeny and function: conventional DC (cDC1 and cDC2), plasmacytoid DC (pDC), and monocyte derived DC (MoDC). DC of Artiodactyla (pigs and ruminants) can also be sub-classified using this system, allowing direct functional and phenotypic comparison of MoDC and other DC subsets trafficking in blood (bDC). Because of the high volume of blood collections required to study DC, cattle offer the best opportunity to further our understanding of bDC and MoDC function in an outbred large animal species. As reported here, phenotyping DC using a monoclonal antibody (mAb) to CD209 revealed CD209 is expressed on the major myeloid population of DC present in blood and MoDC, providing a phenotypic link between these two subsets. Additionally, the present study demonstrates that CD209 is also expressed on monocyte derived macrophages (MoΦ). Functional analysis revealed each of these populations can take up and process antigens (Ags), present them to CD4 and CD8 T cells, and elicit a T-cell recall response. Thus, bDC, MoDC, and MoΦ pulsed with pathogens or candidate vaccine antigens can be used to study factors that modulate DC-driven T-cell priming and differentiation ex vivo.