The Inhibitory Effects of Djulis ( Chenopodium formosanum ) and Its Bioactive Compounds on Adipogenesis in 3T3-L1 Adipocytes
The aim of this study was to provide new insights into the role of the ethanolic extracts of Djulis ( , EECF) and its bioactive compounds in preventing adipogenesis in 3T3-L1 adipocytes. The results demonstrated EECF significantly inhibited oil red O-stained material (OROSM), triglyceride levels and...
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Published in | Molecules (Basel, Switzerland) Vol. 23; no. 7; p. 1780 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
19.07.2018
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | The aim of this study was to provide new insights into the role of the ethanolic extracts of Djulis (
, EECF) and its bioactive compounds in preventing adipogenesis in 3T3-L1 adipocytes. The results demonstrated EECF significantly inhibited oil red O-stained material (OROSM), triglyceride levels and glycerol-3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 adipocytes. The expression of the critical molecules involved in lipid synthesis such as PPARγ, C/EBPα and SREBP-1c was attenuated in EECF-treated cells. According to HPLC-DAD and HPLC-MS/MS analysis, rutin, kaempferol, betanin and another nine compounds were present in EECF. The suppression of lipid accumulation by rutin, kaempferol and betanin occurred by decreasing the gene expression of PPARγ, C/EBPα and SREBP-1c. Taken together, these findings suggest the presence of bioactive compounds in EECF may partly account for the anti-adipogenesis of EECF and EECF is therefore a potentially lipid lowering functional food. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. |
ISSN: | 1420-3049 1420-3049 |
DOI: | 10.3390/molecules23071780 |