A Novel Enzymatic Method for Measuring Mizoribine 5′-Monophosphate Levels in Serum

• Published in: Journal of bioscience and bioengineering Vol. 106; no. 5; pp. 511 - 514
• Main Authors: Ota, Hiroko, Yasuda, Yuko, Sakasegawa, Shin-Ichi, Imamura, Shigeyuki, Tamura, Tomohiro
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.11.2008; Elsevier; Elsevier Limited
• Subjects: ACIDE NUCLEIQUE; ACIDOS NUCLEICOS; ADMINISTRACION ORAL; ADMINISTRATION ORALE; ANALISIS ENZIMATICO; ANALYSE ENZYMATIQUE; Automation; Bacillus - enzymology; BACILLUS SUBTILIS; Bacillus subtilis - enzymology; Biochemistry - methods; Biological and medical sciences; Biotechnology; Biotechnology - methods; Blood Chemical Analysis - instrumentation; Blood Chemical Analysis - methods; BLOOD SERUM; Cations; CLINICAL TRIALS; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; ENSAYOS CLINICOS; ENZYMATIC ANALYSIS; ESSAI CLINIQUE; Fundamental and applied biological sciences. Psychology; Humans; Hydrogen-Ion Concentration; IMMUNODEPRESSEUR; IMMUNOSUPPRESSANTS; Immunosuppressive Agents - pharmacology; IMP Dehydrogenase - chemistry; INMUNODEPRESORES; inosine 5′-monophosphate dehydrogenase; METABOLITE; METABOLITES; METABOLITOS; mizoribine; mizoribine 5′-monophosphate; NUCLEIC ACIDS; Oceanobacillus iheyensis; ORAL ADMINISTRATION; Recombinant Proteins - chemistry; Ribonucleosides - analysis; Ribonucleosides - blood; SERUM SANGUIN; SUERO SANGUINEO; inosine 5′-monophosphate dehydrogenase; MZR; Bacillus subtilis; mizoribine 5′-monophosphate; Oceanobacillus iheyensis; IMP; mizoribine; MZR-P; mizoribine 5'-monophosphate; Enzyme; inosine 5'-monophosphate dehydrogenase; Bacillaceae; Dehydrogenase; Mizoribine; Oceanobacillus iheyensis. Bacillus subtilis; Enzymatic method; Bacillales; Bacteria; Inosine; Serum; Oxidoreductases
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1263/jbb.106.511

Inosine 5′-monophosphate dehydrogenases (IMPDH) from Oceanobacillus iheyensis and Bacillus subtilis were biochemically characterized with the aim of establishing a mizoribine 5′-monophosphate (MZR-P) assay. MZR-P is the active metabolite of mizoribine, which has been successfully used as an immunosuppressive agent. A sensitive method for measuring the MZR-P concentration in serum, based on its ability to inhibit IMPDH, was developed. The method was applied to an automatic clinical analyzer, enabling measurement of MZR-P levels in a set of serum samples. MZR-P at concentrations of 0.1–13.6 (μg/ml (0.3–40 μM) in the samples could be measured with 0.7% within-run coefficients of variation.