Adiposity induced by adenovirus 5 inoculation

• Published in: International Journal of Obesity Vol. 29; no. 6; pp. 603 - 606
• Main Authors: So, P-W, Herlihy, A H, Bell, J D
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.06.2005; Nature Publishing; Nature Publishing Group
• Subjects: Adeno-associated dependoparvovirus B; Adenoviridae; Adenoviridae Infections; Adenoviridae Infections - complications; Adenoviridae Infections - metabolism; Adenoviruses; Adipose tissue; analysis; animal models; Animals; Biological and medical sciences; Body Composition; Body fat; complications; Eating; Epidemiology; Female; Food; food intake; Health Promotion and Disease Prevention; Infections; Injection; Internal Medicine; Lipids; Lipids - analysis; Liver; Liver - metabolism; Magnetic Resonance Spectroscopy; Medical sciences; Medicine; Medicine & Public Health; Metabolic Diseases; metabolism; Mice; Mice, Inbred Strains; Models, Animal; Obesity; Obesity - metabolism; Obesity - virology; Public Health; risk factors; Spectroscopy; Spectrum analysis; Time Factors; vaccination; viral diseases of animals and humans; Viral infections; virology; Viruses; Weight Gain; United Kingdom > UK; bodyweight; adiposity; adenovirus 5; infectobesity; MRS; Obesity; Adenoviridae; Adipose tissue; Body weight; Rodentia; Nutrition disorder; Virus; Adenovirus 5; Vertebrata; Inoculation; Mammalia; Mouse; Human adenovirus; Animal; Mastadenovirus; Biological effect; Nutritional status
• ISSN: 0307-0565; 1476-5497
• DOI: 10.1038/sj.ijo.0802917

OBJECTIVE: To investigate the effect of viral inoculation by adenovirus 5 (Ad5) on body composition in a mouse model. DESIGN: Longitudinal monitoring before and after a single injection of virus or saline. SUBJECTS: Two groups of CD1 mice, one group given a single intraperitoneal dose of Ad5 and the control group, saline. MEASUREMENTS: Bodyweights and food intake were recorded before and up to 21 weeks after inoculation. At the end of the study, whole-body 1 H magnetic resonance spectroscopy (MRS) and localised in vivo 1 H MRS spectroscopy of the liver was performed to assess whole-body adiposity and intrahepatic lipid content, respectively. RESULTS: Ad5-treated animals gained significantly more weight over a period of 21 weeks after inoculation than the controls, 21.8 g (18.8–25.0) and 18.8 g (17.3–19.8) respectively, ( P <0.05). The gain in bodyweight in the former animals arises from increased deposition of adipose tissue as measured by whole-body 1 H MRS. Adiposity was 6.7% (3.10–11.20%), and 2.40% (0.85–5.65%) for the Ad5-treated and control animals, respectively ( P <0.05). No significant difference in intrahepatic lipid content or food intake was observed between the two groups. CONCLUSION: The significantly higher percentage of adipose tissue in the Ad5-treated mice suggest viral infection may play a contributory role to a predisposition to obesity, although its contribution relative to other factors remains to be determined.