Structure Characterization and Hypoglycaemic Activities of Two Polysaccharides from Inonotus obliquus

• Published in: Molecules (Basel, Switzerland) Vol. 23; no. 8; p. 1948
• Main Authors: Liu, Ping, Xue, Jiao, Tong, Shisheng, Dong, Wenxia, Wu, Peipei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.08.2018; MDPI
• Subjects: alpha-Glucosidases - metabolism; Animals; Basidiomycota - chemistry; Cellulose; Chromatography, High Pressure Liquid; Column chromatography; Diabetes mellitus; Enzyme Activation - drug effects; Galactose; Glucose; Glucosidase; Hep G2 Cells; HepG2 cells; High performance liquid chromatography; Humans; Hyperglycemia - drug therapy; Hypoglycemia; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - pharmacology; Hypoglycemic Agents - therapeutic use; In vivo methods and tests; Infrared analysis; Infrared spectra; Inonotus obliquus; Liquid chromatography; Magnetic Resonance Spectroscopy; Mice; Monosaccharides; NMR; Nuclear magnetic resonance; polysaccharide; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - pharmacology; Polysaccharides - therapeutic use; Proteins; Saccharides; Streptozocin; Structural analysis; structure; α-Glucosidase; α-glucosidase inhibitory activity; Inonotus obliquus; HepG2 cells; polysaccharide; α-glucosidase inhibitory activity; structure
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules23081948

In the present study, two polysaccharides (HIOP1-S and HIOP2-S) were isolated and purified from using DEAE-52 cellulose and Sephadex G-100 column chromatography. The structural characterization and in vitro and in vivo hypoglycaemic activities of these molecules were investigated. HPLC analysis HIOP1-S was a heterpolysaccharide with glucose and galactose as the main compontent monosaccharides (50.247%, molar percentages). However, HIOP2-S was a heterpolysaccharide with glucose as the main monosaccharide (49.881%, molar percentages). The average molecular weights of HIOP1-S and HIOP2-S were 13.6 KDa and 15.2 KDa, respectively. The β-type glycosidic bond in HIOP1-S and HIOP2-S was determined using infrared analysis. ¹H-NMR spectra indicated that HIOP2-S contains the β-configuration glycosidic bond, and the glycoside bonds of HIOP1-S are both α-type and β-type. The ultraviolet scanning showed that both HIOP1-S and HIOP2-S contained a certain amount of binding protein. Congo red test showed that HIOP1-S and HIOP2-S could form a regular ordered triple helix structure in the neutral and weakly alkaline range. HIOP1-S and HIOP2-S showed strong α-glucosidase inhibitory activities and increased the glucose consumption of HepG2 cells. In addition, Streptozotocin (STZ)-induced hyperglycaemic mice were used to evaluate the antihyperglycaemic effects of HIOP1-S and HIOP2-S in vivo. The results showed that HIOP2-S had antihyperglycaemic effects. Taken together, these results suggest that HIOP1-S and HIOP2-S have potential anti-diabetic effects.