The A20 cDNA induced by tumor necrosis factor alpha encodes a novel type of zinc finger protein

• Published in: The Journal of biological chemistry Vol. 265; no. 25; pp. 14705 - 14708
• Main Authors: A W Opipari, Jr, M S Boguski, V M Dixit
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 05.09.1990
• Subjects: A20 protein; Amino Acid Sequence; Base Sequence; Biological and medical sciences; Blotting, Southern; Cells, Cultured; DNA - genetics; DNA - isolation & purification; DNA-Binding Proteins - biosynthesis; DNA-Binding Proteins - genetics; Endothelium, Vascular - metabolism; Fundamental and applied biological sciences. Psychology; Gene Library; genes; Genes. Genome; Humans; Metalloproteins - biosynthesis; Metalloproteins - genetics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Plasmids; Sequence Homology, Nucleic Acid; Tumor Necrosis Factor-alpha - pharmacology; Zinc - metabolism; zinc finger protein; Human; Nucleotide sequence; Induction; Zinc finger structure; Sequence alignment; Biosynthesis; Gene expression; Proteins; Complementary DNA; Southern blotting; Computer aid; Tumor necrosis factor α; Aminoacid sequence
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/s0021-9258(18)77165-2

We have previously identified in endothelial cells a gene product designated A20, that is rapidly and profoundly induced by tumor necrosis factor alpha (Dixit, V. M., Green, S., Sarma, V., Holzman, L. B., Wolf, F. W., O'Rourke, K., Ward, P. A., Prochownik, E. V., and Marks, R. M. (1990) J. Biol. Chem. 265, 2973-2978). Isolation of the full-length cDNA followed by sequence analysis has revealed that A20 codes for a novel zinc finger protein. Structural features suggest that this putative protein, which contains multiple Cys2/Cys2 finger motifs, defines a novel class of zinc finger proteins. Southern analysis supports the existence of other members of the class.