Sweat chloride quantification using capillary electrophoresis

• Published in: Practical laboratory medicine Vol. 13; p. e00114
• Main Authors: Dubot, Patricia, Liang, Jing, Dubs, Jacobé, Missiak, Yohann, Sarazin, Cédric, Couderc, François, Caussé, Elizabeth
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2019; Elsevier
• Subjects: Capillary electrophoresis; Chemical Sciences; Chloride; Cystic fibrosis; Sweat test; LOD; ST; CE; CF; CV; Sweat test; Chloride; Capillary electrophoresis; Cystic fibrosis; IS; CFTR; CV, coefficient of variation; ST, sweat test; CF, cystic fibrosis; IS, internal standard; CE, capillary electrophoresis; CFTR, cystic fibrosis transmembrane conductance regulator; LOD, limit of detection
• ISSN: 2352-5517; 2352-5517
• DOI: 10.1016/j.plabm.2018.e00114

Cystic fibrosis (CF) is the less rare and severe genetic disease among the European population. Biochemical diagnosis of CF is based on the demonstration of increased chloride concentration in sweat samples, obtained during the sweat test (ST). WynSep developed a capillary electrophoresis with contactless conductivity detection (CE-C4D) able to measure sweat chloride with a low sample volume. We evaluated the clinical feasibility of this device in a cohort of patients suspected of CF, in comparison with the common coulometric method (ChloroChek chloridometer). We determined sweat chloride concentration of 65 samples from patients referred to our institution to undergo a sweat test. Each sample was submitted to coulometric method first, then WynSep-CE, with or without internal standard (IS) subject to sufficient volume sample. A total of 53 samples were analysed by both coulometric and WynSep-CE (using IS) methods. The method validation showed comparable analytical performances for both methods; no false positive or false negative was recorded. The two methods showed a high correlation (r = 0.993, p < 0.001) and a close agreement was demonstrated by two different statistical tests (Bland-Altman and Passing-Bablok). WynSep-CE is an accurate, fast, easy-to-use and an appropriate method for CF diagnosis.