Circadian renal rhythms influenced by implanted encapsulated hANP-producing cells in Goldblatt hypertensive rats

• Published in: Gene therapy Vol. 11; no. 20; pp. 1515 - 1522
• Main Authors: CHEN, L. G, WANG, Z. R, WAN, C. M, XIAO, J, GUO, L, GUO, H. L, CORNELISSEN, G, HALBERG, F
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing Group 01.10.2004
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Applied cell therapy and gene therapy; Atrial Natriuretic Factor - genetics; Atrial natriuretic peptide; Biocompatible Materials; Biological and medical sciences; Biotechnology; Blood flow; Blood pressure; Cardiovascular diseases; CHO Cells - metabolism; CHO Cells - transplantation; Circadian Rhythm; Circadian rhythms; Cricetinae; Cyclic GMP; Diuresis; Excretion; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene therapy; Genetic Therapy - methods; Glomerular filtration rate; Health. Pharmaceutical industry; Humans; Hypertension; Hypertension, Renovascular - metabolism; Hypertension, Renovascular - therapy; Industrial applications and implications. Economical aspects; Kidney - metabolism; Kidneys; Male; Medical sciences; Models, Animal; Natriuresis; Polycaprolactone; Rats; Rats, Wistar; Renal function; Rodents; Transfection - methods; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Hypertension; Implant; Peptides; Rat; Rodentia; Cardiovascular disease; encapsulated transfected cells; Biological rhythm; Circadian rhythm; Kidney; Vertebrata; Mammalia; Transfection; Urinary system; Gene therapy; atrial natriuretic peptide
• ISSN: 0969-7128; 1476-5462
• DOI: 10.1038/sj.gt.3302330

Renal excretion in experimental hypertensive rats implanted with encapsulated human atrial natriuretic peptide (hANP)-producing cells is circadian periodic. Chinese hamster ovary (CHO) cells transfected with the plasmid hANP-cDNA were encapsulated in biocompatible polycaprolactone capsules for intraperitoneal implantation into two-kidney, one-clip (2K1C) hypertensive rats. During a 12:12 light-dark cycle, as compared to control CHO cells, the implantation of encapsulated hANP-producing CHO cells was associated with an increase in the net excretion of water, sodium and potassium, and with a reversal of the advanced circadian phases related to renovascular hypertension in 2K1C rats. The increase in blood pressure postimplantation was delayed, and increases in renal blood flow, glomerular filtration rate, sodium output, urinary excretion and urinary cyclic GMP concentrations were also found. Implantation of encapsulated hANP-producing cells affects circadian rhythms in kidney excretion functions of 2K1C rats, and may be useful for the treatment of cardiovascular disease.