Sialic Acid-Binding Lectin from Bullfrog Eggs Exhibits an Anti-Tumor Effect Against Breast Cancer Cells Including Triple-Negative Phenotype Cells

• Published in: Molecules (Basel, Switzerland) Vol. 23; no. 10; p. 2714
• Main Authors: Tatsuta, Takeo, Sato, Shoko, Sato, Toshiyuki, Sugawara, Shigeki, Suzuki, Tsuneyoshi, Hara, Akiyoshi, Hosono, Masahiro
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21.10.2018; MDPI
• Subjects: Acids; Amphibian Proteins - chemistry; Amphibian Proteins - pharmacology; Animals; Apoptosis; Apoptosis - drug effects; Binding; Biotechnology; Breast cancer; Cancer therapies; Cell growth; Cell Proliferation - drug effects; Chemotherapy; Eggs; Epidermal growth factor; Epidermal growth factor receptors; ErbB family; ErbB protein; ErbB Receptors - genetics; ErbB-2 protein; Estrogen Receptor alpha - genetics; Estrogen receptors; Estrogens; Female; Gene Expression Regulation, Neoplastic - drug effects; Growth factors; Humans; Kinases; lectin; Lectins - chemistry; Lectins - pharmacology; MAP kinase; MCF-7 Cells; p38 Mitogen-Activated Protein Kinases - genetics; Phenotype; Phenotypes; Phosphorylation; Progesterone; Protein kinase; Proteins; Rana catesbeiana; Receptor, ErbB-2 - genetics; Ribonuclease; Ribonucleases - chemistry; Ribonucleases - pharmacology; sialic acid-binding lectin; Triple Negative Breast Neoplasms - drug therapy; Triple Negative Breast Neoplasms - pathology; Tumor cell lines; Tumors; Western blotting; Japan; breast cancer; sialic acid-binding lectin; lectin; ErbB family; ribonuclease
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules23102714

Sialic acid-binding lectin from eggs (cSBL) is a multifunctional protein that has lectin and ribonuclease activity. In this study, the anti-tumor activities of cSBL were assessed using a panel of breast cancer cell lines. cSBL suppressed the cell growth of all cancer cell lines tested here at a concentration that is less toxic, or not toxic at all, to normal cells. The growth suppressive effect was attributed to the cancer-selective induction of apoptosis. We assessed the expressions of several key molecules associated with the breast cancer phenotype after cSBL treatment by western blotting. cSBL decreased the expression level of estrogen receptor (ER) α, while it increased the phosphorylation level of p38 mitogen-activated protein kinase (MAPK). cSBL also suppressed the expression of the progesterone receptor (PgR) and human epidermal growth factor receptor type 2 (HER2). Furthermore, it was revealed that cSBL decreases the expression of the epidermal growth factor receptor (EGFR/HER1) in triple-negative breast cancer cells. These results indicate that cSBL induces apoptosis with decreasing ErbB family proteins and may have great potential for breast cancer chemotherapy, particularly in triple-negative phenotype cells.