Isolation and Long-term Cultivation of Mouse Alveolar Macrophages

Alveolar macrophages (AM) are tissue-resident macrophages that colonize the lung around birth and can self-maintain long-term in an adult organism without contribution of monocytes. AM are located in the pulmonary alveoli and can be harvested by washing the lungs using the method of bronchoalveolar...

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Bibliographic Details
Published inBio-protocol Vol. 9; no. 14
Main Authors Busch, Clara, Favret, Jérémy, Geirsdóttir, Laufey, Molawi, Kaaweh, Sieweke, Michael
Format Journal Article
LanguageEnglish
Published United States Bio-protocol LCC 20.07.2019
Bio-Protocol
Bio-protocol LLC
Subjects
Biology
Immunology
Life Sciences
Methods
Self-renewal
Alveolar
Lungs
Bronchoalveolar lavage
Macrophage
Primary cell culture
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Summary:Alveolar macrophages (AM) are tissue-resident macrophages that colonize the lung around birth and can self-maintain long-term in an adult organism without contribution of monocytes. AM are located in the pulmonary alveoli and can be harvested by washing the lungs using the method of bronchoalveolar lavage (BAL). Here, we compared different conditions of BAL to obtain high yields of murine AM for culture and expansion of AM. In addition, we describe specific culture conditions, under which AM proliferate long-term in liquid culture in the presence of granulocyte-macrophage colony-stimulating factor. This method can be used to obtain large numbers of AM for transplantation or for experiments with primary mouse macrophages.
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ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.3302